Molecular and General Genetics MGG

, Volume 254, Issue 6, pp 674–680

Flipper, a mobile Fot1-like transposable element in Botrytis cinerea

  • C. Levis
  • D. Fortini
  • Y. Brygoo
ORIGINAL PAPER

DOI: 10.1007/s004380050465

Cite this article as:
Levis, C., Fortini, D. & Brygoo, Y. Mol Gen Genet (1997) 254: 674. doi:10.1007/s004380050465

Abstract

A transposable element, Flipper, was isolated from the phytopathogenic fungus Botrytis cinerea. The element was identified as an insertion sequence within the coding region of the nitrate reductase gene. The Flipper sequence is 1842 bp long with perfect inverted terminal repeats (ITRs) of 48 bp and an open reading frame (ORF) of 533 amino acids, potentially encoding for a transposase; the element is flanked by the dinucleotide TA. The encoded protein is very similar to the putative transposases of three elements from other phytopathogenic fungi, Fot1 from Fusarium oxysporum, and Pot2 and MGR586 from Magnaporthe grisea. The number of Flipper elements in strains of B. cinerea varied from 0 to 20 copies per genome. Analysis of the descendants of one cross showed that the segregation ratio of Flipper elements was 2:2 and that the copies were not linked.

Key words Filamentous fungi   Botrytis cinerea   Transposable element   Fot-1 like   Nitrate reductase 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • C. Levis
    • 1
  • D. Fortini
    • 1
  • Y. Brygoo
    • 1
  1. 1.Station de Pathologie Végétale, INRA, Route de Saint Cyr, 78026 Versailles Cedex, FranceFR

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