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Molecular and General Genetics MGG

, Volume 254, Issue 1, pp 81–84 | Cite as

Molecular cloning and characterisation of the ribC gene from Bacillus subtilis : a point mutation in ribC results in riboflavin overproduction

  • D. Coquard
  • M. Huecas
  • M. Ott
  • J. M. van Dijl
  • A. P. G. M. van Loon
  • H.-P. Hohmann
ORIGINAL PAPER

Abstract

A mutation leading to roseoflavin resistance and deregulated riboflavin biosynthesis was mapped in the genome of the riboflavin-overproducing Bacillus subtilis strains RB52 and RB50 at map position 147°. The chromosomal location indicates that the deregulating mutation in RB52 and RB50 is an allele of the previously identified ribC mutation. We cloned the ribC gene and found that it encodes a putative 36-kDa protein. Surprisingly, RibC has significant sequence similarity to flavin kinases and FAD synthases from various other bacterial species. By comparing the deduced amino acid sequence of RibC from the wild-type parent strain of RB50 with the RibC sequence from the riboflavin-overexpressing RB50 mutant we identified a point mutation that resulted in a Gly to Ser exchange in the C-terminal region of the product

Key wordsBacillus subtilis Riboflavin biosynthesis ribC Flavin kinase FAD synthase 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • D. Coquard
    • 1
  • M. Huecas
    • 1
  • M. Ott
    • 2
  • J. M. van Dijl
    • 3
  • A. P. G. M. van Loon
    • 1
  • H.-P. Hohmann
    • 1
  1. 1.Biotechnology Section of the Vitamin Division (VFCB), Lab. 322/Bldg 93 Hoffmann - La Roche AG Grenzacherstr. 124 4002 Basel, SwitzerlandCH
  2. 2.Pharmaceutical Research - Gene Technologies (PRTP), Hoffmann - La Roche AG, Grenzacherstr. 124, 4002 Basel, SwitzerlandCH
  3. 3.Department of Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, Kerklaan 30, 9751 NN Haren, The NetherlandsNL

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