Molecular and General Genetics MGG

, Volume 254, Issue 1, pp 63–72 | Cite as

Aluminum-sensitive mutants of Saccharomyces cerevisiae

  • E. J. Schott
  • R. C. Gardner
ORIGINAL PAPER

Abstract

We are developing budding yeast, Saccharomyces cerevisiae, as a genetic system for the study of tolerance to the trivalent aluminum cation (Al3+). We have isolated eight mutants that are more sensitive to Al3+ than the wild type. Each mutant represented a different complementation group. A number of the mutants were pleiotropic, and showed defects in other stress responses, changes in tolerance to other metal cations, or abnormal morphology. Two mutants also showed increased dependence on supplemental Mg2+ and Ca2+. One mutant with a relatively specific sensitivity to Al3+ was chosen for molecular complementation. Normal Al3+ tolerance was restored by expression of the MAP kinase gene SLT2. Strains carrying deletions of the SLT2 gene, or of the gene for the corresponding MAP kinase–kinase SLK1, showed sensitivity to Al3+. These results indicate that the SLT2 MAP kinase signal transduction pathway is required for yeast to sense and respond to Al3+ stress.

Key words Yeast   MAP kinase   Protein kinase C    Metal tolerance   Aluminum 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • E. J. Schott
    • 1
  • R. C. Gardner
    • 1
  1. 1.Centre for Gene Technology, School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New ZealandNZ

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