Molecular and General Genetics MGG

, Volume 253, Issue 5, pp 562–567

Effects of the sinR and degU32 (Hy) mutations on the regulation of the aprE gene in Bacillus subtilis

  • J. Olmos
  • R. de Anda
  • E. Ferrari
  • F. Bolívar
  • F. Valle
ORIGINAL PAPER

Abstract

The aprE gene of Bacillus subtilis codes for the serine alkaline protease known as subtilisin. Its expression is regulated by a complex network of activators and repressors that includes the products of hpr, degU and sinR. In order to understand the effect of these gene products on subtilisin expression, strains carrying combinations of the degU32(Hy), hpr2 and sinR null mutations, were constructed. We found that in all the genetic backgrounds tested, the sinR null mutation decreased aprE expression. Also, by measuring alkaline phosphatase synthesis and the formation of heat-resistant spores, as indicators of sporulation, we found that some of the mutant strains showed alterations in the sporulation process. These results suggest that these alterations are partially responsible for some of the observed changes in aprE expression.

Key words Sporulation process Proteases Gene regulation Transition state regulators 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • J. Olmos
    • 1
  • R. de Anda
    • 1
  • E. Ferrari
    • 2
  • F. Bolívar
    • 1
  • F. Valle
    • 1
  1. 1.Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510–3, Cuernavaca, Morelos 62250, México
  2. 2.Genencor International Inc., 925 Page Mill Road, Palo Alto, CA 94304-1013, USAUS

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