Molecular and General Genetics MGG

, Volume 253, Issue 4, pp 520–528

The Aspergillus nidulans genes chsA and chsD encode chitin synthases which have redundant functions in conidia formation

  • T. Motoyama
  • Makoto Fujiwara
  • Nobuko Kojima
  • Hiroyuki Horiuchi
  • Akinori Ohta
  • M. Takagi
ORIGINAL PAPER/CORRIGENDUM
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Abstract

 We previously isolated three chitin synthase genes (chsA, chsB, and chsC) from Aspergillus nidulans. In the present work, we describe the isolation and characterization of another chitin synthase gene, named chsD, from A. nidulans. Its deduced amino acid sequence shows 56.7% and 55.9% amino acid identity, respectively, with Cal1 of Saccharomyces cerevisiae and Chs3 of Candida albicans. Disruption of chsD caused no defect in cell growth or morphology during the asexual cycle and caused no decrease in chitin content in hyphae. However, double disruption of chsA and chsD caused a remarkable decrease in the efficiency of conidia formation, while double disruption of chsC and chsD caused no defect. Thus it appears that chsA and chsD serve redundant functions in conidia formation.

Key words Chitin synthase Multigene family Cell wall Conidia Aspergillus nidulans 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • T. Motoyama
    • 1
  • Makoto Fujiwara
    • 1
  • Nobuko Kojima
    • 1
  • Hiroyuki Horiuchi
    • 1
  • Akinori Ohta
    • 1
  • M. Takagi
    • 1
  1. 1.Department of Biotechnology, The University of Tokyo, Bunkyo-ku, Tokyo 113, JapanJP

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