Molecular and General Genetics MGG

, Volume 253, Issue 1–2, pp 189–197

δ-(L-α-Aminoadipyl)-L-cysteinyl-D-valine synthetase is a rate limiting enzyme for penicillin production in Aspergillusnidulans

  • J. Kennedy
  • G. Turner
ORIGINAL PAPER

Abstract

 The acvA gene from Aspergillus nidulans encoding δ-(L-α-aminoadipyl)-L-cysteinyl-D-valine (ACV) synthetase was overexpressed by replacing the wild-type acvA promoter with the ethanol dehydrogenase promoter, alcAp, from A. nidulans. The expression level of alcAp was determined using a strain in which the reporter gene, lacZ, is under the control of alcAp, and was found to be up to 100 times greater than that from the acvA promoter when induced in fermentation conditions. Penicillin yields were found to increase by as much as 30-fold when the acvA gene was overexpressed. Glucose, which strongly represses transcription from alcAp, also repressed penicillin biosynthesis in the overexpression strain. These results prove that ACV synthetase is a rate limiting enzyme for penicillin production in A. nidulans.

Key words ACVS Penicillin Overexpression Flux control alcA promoter 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • J. Kennedy
    • 1
  • G. Turner
    • 1
  1. 1.Department of Molecular Biology and Biotechnology, Krebs Institute for Biomolecular Research, The University of Sheffield, Sheffield S10 2TN, UKGB

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