Molecular and General Genetics MGG

, Volume 253, Issue 1, pp 32–41

Chromosome landing at the Arabidopsis TORNADO1 locus using an AFLP-based strategy

  • G. Cnops
  • B. den Boer
  • A. Gerats
  • M. Van Montagu
  • M. Van Lijsebettens
ORIGINAL PAPER

DOI: 10.1007/s004380050293

Cite this article as:
Cnops, G., den Boer, B., Gerats, A. et al. Mol Gen Genet (1996) 253: 32. doi:10.1007/s004380050293

Abstract

 The Arabidopsis tornado1 (trn1) mutation causes severe dwarfism combined with twisted growth of all organs. We present a chromosome landing strategy, using amplified restriction fragment length polymorphism (AFLP) marker technology, for the isolation of the TRN1 gene. The recessive trn1 mutation was identified in a C24 transgenic line and is located 5 cM from a T-DNA insertion. We mapped the TRN1 locus to the bottom half of chromosome 5 relative to visible and restriction fragment length polymorphism (RFLP) markers. Recombinant classes within a 3-cM region around TRN1 were used to build a high-resolution map in this region, using the AFLP technique. Approximately 300 primer combinations have been used to test about 26 000 fragments for polymorphisms. Seventeen of these AFLP markers were identified in the 3-cM region around TRN1. These markers were mapped within this region using individual recombinants. Four of these AFLP markers co-segregate with TRN1 whereas one maps at one recombinant below TRN1. We isolated and cloned three of these AFLP markers. These markers identified two yeast artificial chromosome (YAC) clones, containing the RFLP marker above and the AFLP marker below TRN1, demonstrating that these YACs span the TRN1 locus and that chromosome landing has been achieved, using an AFLP-based strategy.

Key words AFLP markers Arabidopsis thaliana Chromosome landing Physical mapping tornado1 

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • G. Cnops
    • 1
  • B. den Boer
    • 1
  • A. Gerats
    • 1
  • M. Van Montagu
    • 1
  • M. Van Lijsebettens
    • 1
  1. 1.Laboratorium voor Genetica, Department of Genetics, Flanders Interuniversity Institute for Biotechnology, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, BelgiumBE

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