Advertisement

Molecular and General Genetics MGG

, Volume 261, Issue 4–5, pp 820–830 | Cite as

Molecular cloning and characterisation of a maize cDNA for a homologue of the large subunit of the eukaryotic initiation factor 3 (eIF3)

  • P. A. Sabelli
  • S. R. Burgess
  • L. Valášek
  • P. R. Shewry
ORIGINAL PAPER

Abstract

In order to identify genes that are specifically expressed in distinct cell populations of the maize root apex, we have constructed PCR-directed cDNA libraries from microdissected populations of cells, and screened them by differential hybridisation. A meristem-specific cDNA was isolated and characterised. This cDNA, termed ZmeIF3A, encodes a protein homologous to the large subunit of the eukaryotic translation Initiation Factor 3 (eIF3), which is an essential multi-protein complex for the initiation of protein synthesis. The ZmeIF3A protein is most similar to the yeast homologue RPG1, lacking the repeated C-terminal domain characteristic of its mammalian counterparts. However, despite this similarity, it fails to replace the RPG1 protein in complementation experiments on yeast mutants. Analysis of gene expression in situ showed that the ZmeIF3A transcript is expressed in the region of the root meristem surrounding the central stele. ZmeIF3A mRNA is also expressed in the young root, the male inflorescence, and the developing cob and seed. In maize, ZmeIF3A is encoded by one or two genomic sequences. This is the first report on the isolation and characterisation of a cDNA from higher plants that encodes a product homologous to a component of the eIF3 complex.

Key words eIF3 Maize Protein synthesis Translation 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • P. A. Sabelli
    • 1
  • S. R. Burgess
    • 2
  • L. Valášek
    • 3
  • P. R. Shewry
    • 2
  1. 1.Institute of Molecular and Cell Biology, National University of Singapore, 30 Medical Drive, Singapore 117609, Republic of SingaporeSG
  2. 2.IACR-Long Ashton Research Station, University of Bristol, Long Ashton, Bristol BS18 9AF, UKGB
  3. 3.Institute of Biochemistry and Molecular Cell Biology, Vienna Biocenter, University of Vienna, Dr. Bohrgasse 9, A-1030 Vienna, AustriaAT

Personalised recommendations