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Molecular and General Genetics MGG

, Volume 264, Issue 6, pp 782–789 | Cite as

Essential genes in proximal 3L heterochromatin of Drosophila melanogaster

  • S. Schulze
  • D. A. R. Sinclair
  • E. Silva
  • K. A. Fitzpatrick
  • M. Singh
  • V. K. Lloyd
  • K. A. Morin
  • J. Kim
  • D. G. Holm
  • J. A. Kennison
  • B. M. Honda
Original Paper

Abstract.

We have further characterized essential loci within the centric heterochromatin of the left arm of chromosome 3 (3L) of Drosophila melanogaster, using EMS, radiation and P element mutagenesis. We failed to find any new essential genes, a result that suggests a lower-than-average gene density in this region. Mutations affecting expression of the most proximal gene [lethal 1, l1 or l(3)80Fj] act as dominant suppressors of Polycomb (Pc), behavior which is consistent with a putative trithorax group (trx-G) gene. The third gene to the left of the centromere [lethal 3, l3 or l(3)80Fh] is likely to correspond to verthandi (vtd), a known trx-G gene that plays a role in the regulation of hedgehog (hh) expression and signalling. The intervening gene [lethal 2, l2 or l(3)80Fi] is required throughout development, and mutant alleles have interesting phenotypes; in various allelic combinations that survive, we observe fertility, bristle, wing, eye and cuticle defects.

Essential heterochromatic genes Position-effect variegation Drosophila melanogaster 

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Copyright information

© Springer-Verlag 2000

Authors and Affiliations

  • S. Schulze
    • 1
  • D. A. R. Sinclair
    • 1
  • E. Silva
    • 1
  • K. A. Fitzpatrick
    • 1
  • M. Singh
    • 1
  • V. K. Lloyd
    • 1
  • K. A. Morin
    • 2
  • J. Kim
    • 2
  • D. G. Holm
    • 2
  • J. A. Kennison
    • 3
  • B. M. Honda
    • 1
  1. 1.Department of Molecular Biology and Biochemistry, Simon Fraser University Burnaby, B.C., V5A 1S6 Canada
  2. 2.Department of Zoology, University of British Columbia, Vancouver, BC V6 T 1Z4 Canada
  3. 3.Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892-2785, USA

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