Molecular and General Genetics MGG

, Volume 263, Issue 5, pp 812–827 | Cite as

MUS81 encodes a novel Helix-hairpin-Helix protein involved in the response to UV- and methylation-induced DNA damage in Saccharomyces cerevisiae

  • H. Interthal
  • W.-D. Heyer
ORIGINAL PAPER

Abstract

The gene MUS81 (Methyl methansulfonate, UV sensitive) was identified as clone 81 in a two-hybrid screen using the Saccharomyces cerevisiae Rad54 protein as a bait. It encodes a novel protein with a predicted molecular mass of 72,316 (632 amino acids) and contains two helix-hairpin-helix motifs, which are found in many proteins involved in DNA metabolism in bacteria, yeast, and mammals. Mus81p also shares homology with motifs found in the XPF endonuclease superfamily. Deletion of MUS81 caused a recessive methyl methansulfonate- and UV-sensitive phenotype. However, mus81Δ cells were not significantly more sensitive than wild-type to γ-radiation or double-strand breaks induced by HO endonuclease. Double mutant analysis suggests that Rad54p and Mus81p act in one pathway for the repair of, or tolerance to, UV-induced DNA damage. A complex containing Mus81p and Rad54p was identified in immunoprecipitation experiments. Deletion of MUS81 virtually eliminated sporulation in one strain background and reduced sporulation and spore viability in another. Potential homologs of Mus81p have been identified in Schizosaccharomyces pombe, Caenorhabditis elegans and Arabidopsis thaliana. We hypothesize that Mus81p plays a role in the recognition and/or processing of certain types of DNA damage (caused by UV and MMS) during repair or tolerance processes involving the recombinational repair pathway.

Key words DNA repair Helix-hairpin-Helix motif Methylmethane sulfonate (MMS) Saccharomyces cerevisiae UV radiation 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • H. Interthal
    • 1
  • W.-D. Heyer
    • 1
  1. 1.Institute of General Microbiology, University of Bern, Baltzer-Str. 4, 3012 Bern, SwitzerlandCH
  2. 2.Division of Biological Sciences, Sections of Microbiology, and Molecular and Cellular Biology, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA E-mail: wdheyer@ucdavis.edu Tel.: +1-530-7523001; Fax: +1-530-7523011US

Personalised recommendations