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Genetic mapping of ionomic quantitative trait loci in rice grain and straw reveals OsMOT1;1 as the putative causal gene for a molybdenum QTL qMo8

  • Chengcheng Wang
  • Zhong Tang
  • Jie-Yun Zhuang
  • Zhu Tang
  • Xin-Yuan HuangEmail author
  • Fang-Jie Zhao
Original Article
  • 8 Downloads

Abstract

Rice is a major dietary source of essential mineral nutrients and toxic elements (aka ionome) for humans. However, the genetic basis underlying the variation in ionome is still largely unknown. Here, we mapped 51 and 61 quantitative trait loci (QTLs) controlling the concentrations of 13 and 15 elements in rice (Oryza sativa L.) grain and straw, respectively, using a recombinant inbred lines (RILs) that were grown at three different field sites in 3 years. Several QTLs were repeatedly detected in both grain and straw or in multiple years; the resulting 87 unique QTLs with 17 of them (20%) were co-localized with previously reported corresponding QTLs and 70 were novel ionomic QTLs. At least, 14 genomic clusters that controlled the concentrations of multiple elements were identified. Furthermore, we identified a molybdate transporter gene OsMOT1;1 as the putative causal gene for a QTL controlling molybdenum concentration in both straw and grain. QTL analyses based on the concentrations of multiple elements in both grain and straw of RIL population grown in three field sites in 3 years allow us to identify tissue common QTLs and reproducible QTLs that were validated in multiple years. The identification of ionomic QTLs will be useful in revealing the molecular mechanisms underlying the accumulation of elements in rice and providing the opportunity to reduce the accumulation of toxic elements and enrich the accumulation of beneficial elements in rice grain.

Keywords

RICE Quantitative trait locus Ionome Molybdenum Cadmium Arsenic 

Abbreviations

As

Arsenic

B

Boron

Ca

Calcium

CIM

Composite interval mapping

Cd

Cadmium

cM

Centimorgan

Co

Cobalt

Chr

Chromosome

Cu

Copper

HD

Heading date

ICP-MS

Inductively coupled plasma mass spectrometry

K

Potassium

LOD

Logarithm of the odds

Mg

Magnesium

Mn

Manganese

Mo

Molybdenum

Na

Sodium

Ni

Nickel

P

Phosphorus

Pb

Lead

QTL

Quantitative trait locus

RIL

Recombinant inbred line

Zn

Zinc

Notes

Author contributions

F-JZ and X-YH conceived and designed research, CW, Zhong T and Zhu T conducted experiments, J-YZ provided the RIL population, and X-YH, F-JZ and CW wrote the manuscript with contribution from J-YZ. All authors read and approved the manuscript.

Funding

The work was supported by the National Key Research and Development Program of China (Grant No. 2018YFD0800206), the National Natural Science Foundation of China (Grant No. 31772382, 31520103914), the Innovative Research Team Development Plan of the Ministry of Education of China (Grant No. IRT_17R56), the Fundamental Research Funds for the Central Universities (Grant No. KYT201802) and the Natural Science Foundation of Jiangsu Province for Distinguished Young Scholars (Grant No. KB20180023).

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

This study did not involve human participants or animals.

Supplementary material

438_2019_1632_MOESM1_ESM.pdf (1.5 mb)
Supplementary material 1 (PDF 1533 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental SciencesNanjing Agricultural UniversityNanjingChina
  2. 2.State Key Laboratory of Rice Biology and Chinese National Center for Rice ImprovementNational Rice Research Institute ChinaHangzhouChina

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