Transcriptome sequencing and functional analysis of Sedum lineare Thunb. upon salt stress
Soil salinization is one major constraint to plant geographical distribution, yield, and quality, and as an ideal plant for the “greening” of flat-roofed buildings, Sedum lineare Thunb. has strong tolerance against a variety of environmental adversities including salinity with the underlying mechanism still remaining unknown. In this study, we performed de novo transcriptome sequencing on leaf and root samples of NaCl-treated S. lineare Thunb. and identified 584 differentially expressed genes (DEGs), which were further annotated by gene function classification and pathway assignments using the public data repositories. In addition to the increased gene expression level verified by qRT-PCR, the elevated activities of the corresponding enzymes were also demonstrated for peroxidase (POD), glutathione peroxidases (GPX), and cysteine synthase (CSase) in the NaCl-treated roots. Furthermore, two highly inducible genes without known functions related to salt tolerance were selected to be overexpressed and tested for their effects on salt tolerance in the model plant, Arabidopsis thaliana. Upon 150 mM NaCl treatment, 35S:SlCXE but not 35S:SlCYP72A transgenic Arabidopsis seedlings exhibited improved salt resistance as shown by the increased seed germination rates and longer primary roots of transgenic seedlings when compared to wild-type plants. Taken together, this work laid a foundation for a better understanding of the salt adaptation mechanism of S. lineare Thunb. and genes identified could serve as useful resources for the development of more salt-tolerant varieties of other species through genetic engineering.
KeywordsSalt stress Sedum lineare Thunb. Transcriptome Enzymatic activities Transgenic Arabidopsis
There is no financial support for this work.
In this work, all authors conceived and designed the experiments. XY performed the experiments and analyzed the data. JW and XY wrote the manuscript. All authors read and approved the final manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare no conflicts of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- Flowers TJ, Colmer TD (2010) Salinity tolerance in halophytes. New Phytol 179:945–963Google Scholar
- Forouhar F, Yang Y, Kumar D, Chen Y, Fridman E, Park SW, Chiang Y, Acton TB, Montelione GT, Pichersky E, Klessig DF, Tong L (2005) Structural and biochemical studies identify tobacco SABP2 as a methyl salicylate esterase and implicate it in plant innate immunity. Proc Natl Acad Sci USA 102(5):1773–1778PubMedGoogle Scholar
- Kaleem F, Shabir G, Aslam K, Rasul S, Manzoor H, Shah SM, Khan AR (2018) An overview of the genetics of plant response to salt stress: present status and the way forward. Appl Biochem Biotech 186:1–29Google Scholar
- Le Guan MSH, Nadeem Khan, Maazullah Nasim, Songtao Jiu, Muhammad Fiaz, Xudong Zhu, Kekun Zhang and Jinggui Fang (2018) Transcriptome sequence analysis elaborates a complex defensive mechanism of grapevine (Vitis vinifera L.) in response to salt stress. Int J Mol Sci 19:E4019. https://doi.org/10.3390/ijms19124019 PubMedCentralGoogle Scholar
- Li H, Gao Y, Dai Y, Deng D, Chen J (2013) ZmWRKY33, a WRKY maize transcription factor conferring enhanced salt stress tolerances in Arabidopsis. Plant Growth Regul 70:207–216Google Scholar
- Mansour MMF, Ali EF (2017) Glycinebetaine in saline conditions: an assessment of the current state of knowledge. Acta Physiol Plant 39:56Google Scholar
- Munns R, Tester M (2008) Mechanisms of salinity tolerance. Ann Rev Plant Biol 59:651–681Google Scholar
- Ricardo M, Ricardo S (2017) Soil salinity: effect on vegetable crop growth Management practices to prevent and mitigate soil salinization. Horticulturae 13:30Google Scholar
- Robinson MD, Mccarthy DJ, Smyth GK (2010) edgeR: a bioconductor package for differential expression analysis of digital gene expression data. Bioinformatics 26:139–140Google Scholar
- Shan Q, Liu X, Zhang J, Chen G, Liu S, Zhang P, Ying W (2011) Analysis on the tolerance of four ecotype plants against copper stress in soil. Procedia Environ Sci 10:1802–1810Google Scholar
- Shi W, Liu D, Hao L, Wu CA, Guo X, Li H (2014) GhWRKY39, a member of the WRKY transcription factor family in cotton, has a positive role in disease resistance and salt stress tolerance. Plant Cell Tiss Org 118:17–32Google Scholar
- Shigeto J, Tsutsumi Y (2016) Diverse functions and reactions of class III peroxidases. New Phytol 209:1395–1402Google Scholar
- Tuteja N (2007) Mechanisms of high salinity tolerance in plants. Method Enzymol 428:419–438Google Scholar
- Warrilow AGS, Hawkesford MJ (1998) Separation, subcellular location and influence of sulphur nutrition on isoforms of cysteine synthase in spinach. J Exp Bot 49:1625–1636Google Scholar
- Zhai CZ, Zhao L, Yin LJ, Chen M, Wang QY, Li LC, Xu ZS, Ma YZ (2013) Two wheat glutathione peroxidase genes whose products are located in chloroplasts improve salt and H2O2 tolerances in Arabidopsis. PLoS One 8:e73989. https://doi.org/10.1371/journal.pone.0073989 CrossRefPubMedPubMedCentralGoogle Scholar
- Zhu JK (2002) Salt and drought stress signal transduction in plants. Ann Rev Plant Biol 53:247–273Google Scholar
- Zhu JY, Shi X, Lu H, Xia B, Li Y, Li X, Zhang Q, Yang G (2016) RNA-seq transcriptome analysis of extensor digitorum longus and soleus muscles in large white pigs. Mol Genet Genom 291(2):687–701Google Scholar