QTL analysis of nodule traits and the identification of loci interacting with the type III secretion system in soybean
Symbiotic nitrogen fixation is the main source of nitrogen for soybean growth. Since the genotypes of rhizobia and soybean germplasms vary, the nitrogen-fixing ability of soybean after inoculation also varies. A few studies have reported that quantitative trait loci (QTLs) control biological nitrogen fixation traits, even soybean which is an important crop. The present study reported that the Sinorhizobium fredii HH103 gene rhcJ belongs to the tts (type III secretion) cluster and that the mutant HH103ΩrhcJ can clearly decrease the number of nodules in American soybeans. However, few QTLs of nodule traits have been identified. This study used a soybean (Glycine max (L.) Merr.) ‘Charleston’ × ‘Dongnong 594’ (C × D, n = 150) recombinant inbred line (RIL). Nodule traits were analysed in the RIL population after inoculation with S. fredii HH103 and the mutant HH103ΩrhcJ. Plants were grown in a greenhouse with a 16-h light cycle at 26 °C and an 8-h dark cycle at 18 °C. Then, 4 weeks after inoculation, plants were harvested for evaluation of nodule traits. Through QTL mapping, 16 QTLs were detected on 8 chromosomes. Quantitative PCR (qRT-PCR) and RNA-seq analysis determined that the genes Glyma.04g060600, Glyma.18g159800 and Glyma.13g252600 might interact with rhcJ.
KeywordsSoybean QTL HH103ΩrhcJ Nodule number Nodule dry weight
Financial support was received from the University Project of Young Scientist (UNPYSCT-2015011), the Foundation for University Key Teachers from the Education Department of Heilongjiang Province in China (Grant number: 1254G011), the National Natural Science Foundation of China (Grant numbers: 31400074, 31471516, 31271747, and 30971809), the Natural Science Foundation of Heilongjiang Province of China (Grant number: ZD201213), the Heilongjiang Postdoctoral Science Foundation (Grant number: LBH-Q16014), the Harbin Science Technology Project (Grant numbers: 2013RFQXJ005 and 2014RFXXJ012), the Ministry of Science and Technology of the People’s Republic of China Project (Grant number: 2017YFE0111000), and the National Key R&D Program of China (Grant numbers: 2016YFD0100500, 2016YFD0100300, and 2016YFD0100201-21).
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Conflict of interest
The authors declare that they have no conflict of interest.
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