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Molecular Genetics and Genomics

, Volume 294, Issue 6, pp 1375–1383 | Cite as

Overexpression of BmFoxO inhibited larval growth and promoted glucose synthesis and lipolysis in silkworm

  • Zhongyan Lu
  • Zhu Meng
  • Maoyu Wen
  • Xiaoli Kang
  • Yan Zhang
  • Qingsong Liu
  • Ping Zhao
  • Qingyou XiaEmail author
Original Article

Abstract

Forkhead box O (FoxO) is a downstream transcription factor of the insulin-signaling pathway, which plays vital roles in the growth and metabolism of organisms. In this study, BmFoxO was overexpressed in BmE cells, in which proliferation was inhibited and apoptosis was increased. The transgenic vector overexpressing BmFoxO was constructed, and the transgenic silkworm line A4FoxO was generated via embryonic microinjection. The body size of A4FoxO silkworm was smaller than that of non-transgenic silkworm (WT). The quantitative polymerase chain reaction results revealed that the insulin pathway was enhanced and the growth-related TOR pathway was suppressed. Furthermore, the translation of proteins in the fat body of A4FoxO silkworm was inhibited. The expression level of genes involved in the glucose synthesis and lipolysis pathways was increased, whereas that of genes involved in fat synthesis was decreased. Oil red O staining revealed that the amount of lipid droplets was reduced in A4FoxO silkworms compared with WT. Further analysis showed that the content of triglyceride and glycogen was significantly decreased in fat body, but the content of glucose and trehalose was increased in the hemolymph of A4FoxO silkworms. These results suggest that the enhanced expression of BmFoxO disturbs glycolipid metabolism and affects silkworm growth.

Keywords

BmFoxO Silkworm Overexpression Transgenic Growth Metabolism 

Notes

Acknowledgements

This study was supported National Science Foundation of China (31501910) and the Fundamental Research Funds for the Central Universities (XDJK2019B026). We thank Zhaoming Dong and Liang Jiang for careful modifying of the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare no competing interests in relation to the present work.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Supplementary material

438_2019_1550_MOESM1_ESM.docx (516 kb)
Supplementary material 1 (DOCX 516 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Zhongyan Lu
    • 1
    • 2
  • Zhu Meng
    • 1
  • Maoyu Wen
    • 1
  • Xiaoli Kang
    • 1
  • Yan Zhang
    • 1
    • 2
  • Qingsong Liu
    • 2
  • Ping Zhao
    • 2
    • 3
  • Qingyou Xia
    • 2
    • 3
    Email author
  1. 1.College of BiotechnologySouthwest UniversityChongqingChina
  2. 2.Biological Science Research CenterSouthwest UniversityChongqingChina
  3. 3.Chongqing Engineering and Technology Research Center for Novel Silk MaterialsSouthwest UniversityChongqingChina

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