Over-expression of the red plant gene R1 enhances anthocyanin production and resistance to bollworm and spider mite in cotton
- 75 Downloads
Anthocyanins are a class of pigments ubiquitously distributed in plants and play roles in adoption to several stresses. The red plant gene (R1) promotes light-induced anthocyanin accumulation and red/purple pigmentation in cotton. Using 11 markers developed via genome resequencing, the R1 gene was located in an interval of approximately 136 kb containing three annotated genes. Among them, a PAP1 homolog, GhPAP1D (Gohir.D07G082100) displayed differential transcript level in the red- and green-plant leaves. GhPAP1D encoded a R2R3-MYB transcription factor and its over-expression resulted in increased anthocyanin accumulation in transgenic tobaccos and cottons. Dual luciferase assay indicated that GhPAP1D activated the promoters of several cotton anthocyanin structural genes in tobacco leaves. Importantly, we found that the GhPAP1D-overexpressing cotton leaves had increased resistance to both bollworm and spite mite. Our data demonstrated that GhPAP1D was the controlling gene of the red plant phenotype in cotton, and as the major anthocyanin regulator, this gene was potential to create transgenic cottons with resistance to a broad spectrum of herbivores.
KeywordsAnthocyanin Bollworm Cotton Herbivore resistance Red plant R1 gene R2R3-MYB Spider mite Transcription factor
This study was partially funded by the Genetically Modified Organisms Breeding Major Project of China (2016ZX08005005-001 to Y.H.X.), the National Natural Science Foundation of China (31571582 and 31871539 to Y.H.X.) and the Chongqing Project of Basic and Frontier Research (cstc2015jcyjA80001 to Y.H.X.).
Compliance with ethical standards
Conflict of interest
All the authors declare that they have no conflict of interest.
Human/animal rights statement
This article does not contain any studies with human participants performed by any of the authors.
- Andres RJ, Coneva V, Frank MH, Tuttle JR, Samayoa LF, Han SW, Kaur B, Zhu L, Fang H, Bowman DT, Rojas-Pierce M, Haigler CH, Jones DC, Holland JB, Chitwood DH, Kuraparthy V (2017) Modifications to a LATE MERISTEM IDENTITY1 gene are responsible for the major leaf shapes of Upland cotton (Gossypium hirsutum L.). Proc Natl Acad Sci USA 114:E57–E66CrossRefGoogle Scholar
- Ballester A-R, Molthoff J, de Vos R, Hekkert BtL, Orzaez D, Fernandez-Moreno J-P, Tripodi P, Grandillo S, Martin C, Heldens J, Ykema M, Granell A, Bovy A (2010) Biochemical and molecular analysis of pink tomatoes: deregulated expression of the gene encoding transcription factor SlMYB12 leads to pink tomato fruit color. Plant Physiol 152:71–84CrossRefGoogle Scholar
- Liu D, Liu F, Shan X, Zhang J, Tang S, Fang X, Liu X, Wang W, Tan Z, Teng Z, Zhang Z, Liu D (2015) Construction of a high-density genetic map and lint percentage and cottonseed nutrient trait QTL identification in upland cotton (Gossypium hirsutum L.). Mol Genet Genom 290:1683–1700CrossRefGoogle Scholar
- Nakabayashi R, Yonekura-Sakakibara K, Urano K, Suzuki M, Yamada Y, Nishizawa T, Matsuda F, Kojima M, Sakakibara H, Shinozaki K, Michael AJ, Tohge T, Yamazaki M, Saito K (2014) Enhancement of oxidative and drought tolerance in Arabidopsis by overaccumulation of antioxidant flavonoids. Plant J 77:367–379CrossRefGoogle Scholar
- Yan Q, Wang Y, Li Q, Zhang Z, Ding H, Zhang Y, Liu H, Luo M, Liu D, Song W, Liu H, Yao D, Ouyang X, Li Y, Li X, Pei Y, Xiao Y (2018) Up-regulation of GhTT2-3A in cotton fibres during secondary wall thickening results in brown fibres with improved quality. Plant Biotechnol J 16:1735–1747CrossRefGoogle Scholar