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Molecular Genetics and Genomics

, Volume 294, Issue 1, pp 111–120 | Cite as

An efficient method for multigene co-interference by recombinant Bombyx mori nucleopolyhedrovirus

  • Hao Zheng
  • Feifei Ren
  • Qiuyuan Lu
  • Zhenming Cao
  • Jichen Song
  • Min FengEmail author
  • Jisheng Liu
  • Jingchen SunEmail author
Original Article
  • 119 Downloads

Abstract

Bombyx mori Nucleopolyhedrovirus (BmNPV), which is a member of the Baculoviridae family, is a significant pathogen of the silkworm. The infection of BmNPV is often lethal and causes about 20% loss of cocoon in the silk industry annually. To explore the effects of different gene inhibition strategies on the replication cycle of baculovirus, we constructed the mutant virus to infect BmN cells directly and further identified ie0, ie1, and gp64 as the essential viral genes of BmNPV. To elucidate the significance of the inhibition effect of different interference strategies, we characterized and constructed the recombinant BmNPV that carried a single or multigene-interfering cassette. The results showed that the inhibition effect of dsie1 on target gene expression, virus titer, and silkworm mortality was significantly better than that of dsie0 and dsgp64. It also showed that the dsie1 interference produced fewer progeny virions and was less lethal, which indicates that ie1 played a more critical role in the BmNPV replication cycle. Furthermore, the inhibitory effect of the virus titer and mortality indicated that the multigene co-interference constructed by the baculovirus expression system was significantly better than the interference of any single-gene (p < 0.05). In summary, the strategy of multigene synergy can achieve the function of continuous interference and provide a new platform for the breeding of silkworm disease resistant. In addition, this strategy improves the various traits of the silkworm.

Keywords

Bombyx mori Nucleopolyhedrovirus Multigene Co-interference Gene expression 

Notes

Acknowledgements

This study was funded by the National Natural Science Foundation of China (Grant Nos. 31872426, 31372373), the Natural Science Foundation of Guangdong Province, China (Grant No. 2016A030311018).

Author contributions

HZ and JS (J Sun) coordinated the project. HZ and FR performed the research. HZ JS (J Song) and JS (J Sun) wrote the manuscript. QL and JS (J Sun) contributed new methods and improved the manuscript. QL, ZC, and MF performed the data analysis. HZ, JL, and JS (J Sun) interpreted the context of results. All authors have read and approved the manuscript.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Hao Zheng
    • 1
  • Feifei Ren
    • 1
  • Qiuyuan Lu
    • 1
  • Zhenming Cao
    • 1
  • Jichen Song
    • 2
  • Min Feng
    • 1
    Email author
  • Jisheng Liu
    • 3
  • Jingchen Sun
    • 1
    Email author
  1. 1.Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding and Subtropical Sericulture and Mulberry Resources Protection and Safety Engineering Research Center, College of Animal ScienceSouth China Agricultural UniversityGuangzhouChina
  2. 2.Department of Animal SciencesUniversity of ManitobaWinnipegCanada
  3. 3.School of Life SciencesGuangzhou UniversityGuangzhouChina

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