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Molecular Genetics and Genomics

, Volume 294, Issue 1, pp 35–45 | Cite as

Interspecific genetic maps in Miscanthus floridulus and M. sacchariflorus accelerate detection of QTLs associated with plant height and inflorescence

  • Chunxia Ge
  • Xin Ai
  • Shengfeng Jia
  • Yinqing Yang
  • Lu Che
  • Zili Yi
  • Cuixia ChenEmail author
Original Article

Abstract

Miscanthus is recognized as a promising lignocellulosic crop for the production of bioethanol and bioproducts worldwide. To facilitate the identification of agronomical important traits and establish genetics knowledge, two genetic maps were developed from a controlled interspecific cross between M. floridulus and M. sacchariflorus. A total of 650 SSR markers were mapped in M. floridulus, spanning 19 linkage groups and 2053.31 cM with an average interval of 3.25 cM. The map of M. sacchariflorus comprised 495 SSR markers in 19 linkage groups covering 1684.86 cM with an average interval of 3.54 cM. The estimation on genome length indicated that the genome coverage of parental genetic maps were 93.87% and 89.91%, respectively. Eighty-eight bi-parental common markers were allowed to connect the two maps, and six pairs of syntenic linkage groups were recognized. Furthermore, quantitative trait loci (QTL) mapping of three agronomic traits, namely, plant height (PH), heading time (HT), and flowering time (FT), demonstrated that a total of 66 QTLs were identified in four consecutive years using interval mapping and multiple-QTL model. The LOD value of these QTLs ranged from 2.51 to 10.60, and the phenotypic variation explained varied from 9.50 to 37.10%. QTL cluster in syntenic groups MF19/MS7 contained six stable QTLs associated with PH, HT, and FT. In conclusion, we report for the first time the genetic mapping of biomass traits in M. floridulus and M. sacchariflorus. These results will be a valuable genetic resource, facilitating the discovery of essential genes and breeding of Miscanthus.

Keywords

Miscanthus Genetic map SSR QTL mapping Biomass 

Notes

Acknowledgements

This work was supported by the National Natural Sciences Foundation of China (31271352 and 31071471).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical approval

The authors declare that this study complies with the current laws of the country in which the experiments were performed. This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Chunxia Ge
    • 1
    • 2
  • Xin Ai
    • 4
  • Shengfeng Jia
    • 1
    • 2
  • Yinqing Yang
    • 1
    • 2
  • Lu Che
    • 3
  • Zili Yi
    • 5
  • Cuixia Chen
    • 1
    • 2
    Email author
  1. 1.State Key Laboratory of Crop BiologyShandong Agricultural UniversityTaianChina
  2. 2.College of AgronomyShandong Agricultural UniversityTaianChina
  3. 3.Network Information Technology CenterShandong Agricultural UniversityTaianChina
  4. 4.College of Horticulture and LandscapeHunan Agricultural UniversityChangshaChina
  5. 5.College of Bioscience and BiotechnologyHunan Agricultural UniversityChangshaChina

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