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Developing japonica rice introgression lines with multiple resistance genes for brown planthopper, bacterial blight, rice blast, and rice stripe virus using molecular breeding

  • Russell Reinke
  • Suk-Man Kim
  • Bo-Kyeong Kim
Original Article
  • 48 Downloads

Abstract

Yield losses as a result of biotic stresses by fungi, bacteria, viruses, and insects are a key challenge in most rice cultivation areas. The development of resistant cultivars is considered an efficient and sustainable approach to mitigate rice yield reduction. In the present study, we describe the development of japonica rice introgression lines with multiple resistance genes (MR lines), resistant to four different types of biotic stresses, and compare the agronomic performance, yield, and grain quality parameters of these lines with those of the recurrent parent. A total of nine MR lines were developed by marker-assisted backcrossing, which combined five single-R genes in a japonica background with a minimum of linkage drag. All the MR lines harbored the R genes Bph18 and qSTV11SG and two Pi genes (Pib + Pik) in common, offering resistance to brown planthopper (BPH), rice stripe virus (RSV), and rice blast disease, respectively. In the case of bacterial blight (BB), Xa40 was detected in only five out of the nine and Xa3 was validated in the others. In particular, the five MR lines pyramiding the R genes (Bph18 + qSTV11SG + Pib + Pik) in combination with Xa40 showed stable resistance to all bioassays for BPH, BB, blast, and RSV. The MR lines did not show any negative effects on the main agronomic traits, including yield production and rice grain quality. The lines have significant potential to stabilize rice yield and minimize production costs in disease and pest-prone areas in Korea, through the pyramiding of five R genes using a marker-assisted backcrossing strategy.

Keywords

Multiple resistance genes Biotic stress Gene pyramiding Japonica rice Marker-assisted selection 

Abbreviations

ADV

Alkali digestion value

BB

Disease bacterial blight

BPH

Brown planthopper

CL

Culm length

DH

Days to heading

GW

Grain weight (GW)

MAS

Marker-assisted selection

NGP

Number of filled grains per panicle

NP

Number of panicles

PL

Panicle length

RP

Recurrent parent

RGSV

Rice grassy stunt virus

RRSV

Rice ragged stunt virus

RSV

Rice stripe virus

SS

Seed set rate

Xoo

Xanthomonas oryzae pv. oryzae

Notes

Acknowledgements

This research was based on germplasm developed by Dr. K.K. Jena and was supported in part by a grant to the Agenda Program (Code No. PJ00926202), the Temperate Rice Research Consortium (TRRC) of the Rural Development Administration, Republic of Korea, and the Global Rice Science Partnership (GRiSP) program of IRRI. We thank the IRRI communication team for carefully editing the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest.

Ethical standards

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

438_2018_1470_MOESM1_ESM.docx (38 kb)
Supplementary material 1 (DOCX 37 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Strategic Innovation PlatformInternational Rice Research InstituteMetro ManilaPhilippines
  2. 2.C/o IRRI-Korea Office, National Institute of Crop Science, Rural Development AdministrationIseo-myeon, Wanju-gunRepublic of Korea
  3. 3.National Institute of Crop Science, Rural Development AdministrationIseo-myeon, Wanju-gunRepublic of Korea

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