Developing japonica rice introgression lines with multiple resistance genes for brown planthopper, bacterial blight, rice blast, and rice stripe virus using molecular breeding
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Yield losses as a result of biotic stresses by fungi, bacteria, viruses, and insects are a key challenge in most rice cultivation areas. The development of resistant cultivars is considered an efficient and sustainable approach to mitigate rice yield reduction. In the present study, we describe the development of japonica rice introgression lines with multiple resistance genes (MR lines), resistant to four different types of biotic stresses, and compare the agronomic performance, yield, and grain quality parameters of these lines with those of the recurrent parent. A total of nine MR lines were developed by marker-assisted backcrossing, which combined five single-R genes in a japonica background with a minimum of linkage drag. All the MR lines harbored the R genes Bph18 and qSTV11SG and two Pi genes (Pib + Pik) in common, offering resistance to brown planthopper (BPH), rice stripe virus (RSV), and rice blast disease, respectively. In the case of bacterial blight (BB), Xa40 was detected in only five out of the nine and Xa3 was validated in the others. In particular, the five MR lines pyramiding the R genes (Bph18 + qSTV11SG + Pib + Pik) in combination with Xa40 showed stable resistance to all bioassays for BPH, BB, blast, and RSV. The MR lines did not show any negative effects on the main agronomic traits, including yield production and rice grain quality. The lines have significant potential to stabilize rice yield and minimize production costs in disease and pest-prone areas in Korea, through the pyramiding of five R genes using a marker-assisted backcrossing strategy.
KeywordsMultiple resistance genes Biotic stress Gene pyramiding Japonica rice Marker-assisted selection
Alkali digestion value
Disease bacterial blight
Days to heading
Grain weight (GW)
Number of filled grains per panicle
Number of panicles
Rice grassy stunt virus
Rice ragged stunt virus
Rice stripe virus
Seed set rate
Xanthomonas oryzae pv. oryzae
This research was based on germplasm developed by Dr. K.K. Jena and was supported in part by a grant to the Agenda Program (Code No. PJ00926202), the Temperate Rice Research Consortium (TRRC) of the Rural Development Administration, Republic of Korea, and the Global Rice Science Partnership (GRiSP) program of IRRI. We thank the IRRI communication team for carefully editing the manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that there are no conflicts of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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