Molecular Genetics and Genomics

, Volume 293, Issue 6, pp 1565–1575 | Cite as

Developing japonica rice introgression lines with multiple resistance genes for brown planthopper, bacterial blight, rice blast, and rice stripe virus using molecular breeding

  • Russell ReinkeEmail author
  • Suk-Man Kim
  • Bo-Kyeong Kim
Original Article


Yield losses as a result of biotic stresses by fungi, bacteria, viruses, and insects are a key challenge in most rice cultivation areas. The development of resistant cultivars is considered an efficient and sustainable approach to mitigate rice yield reduction. In the present study, we describe the development of japonica rice introgression lines with multiple resistance genes (MR lines), resistant to four different types of biotic stresses, and compare the agronomic performance, yield, and grain quality parameters of these lines with those of the recurrent parent. A total of nine MR lines were developed by marker-assisted backcrossing, which combined five single-R genes in a japonica background with a minimum of linkage drag. All the MR lines harbored the R genes Bph18 and qSTV11SG and two Pi genes (Pib + Pik) in common, offering resistance to brown planthopper (BPH), rice stripe virus (RSV), and rice blast disease, respectively. In the case of bacterial blight (BB), Xa40 was detected in only five out of the nine and Xa3 was validated in the others. In particular, the five MR lines pyramiding the R genes (Bph18 + qSTV11SG + Pib + Pik) in combination with Xa40 showed stable resistance to all bioassays for BPH, BB, blast, and RSV. The MR lines did not show any negative effects on the main agronomic traits, including yield production and rice grain quality. The lines have significant potential to stabilize rice yield and minimize production costs in disease and pest-prone areas in Korea, through the pyramiding of five R genes using a marker-assisted backcrossing strategy.


Multiple resistance genes Biotic stress Gene pyramiding Japonica rice Marker-assisted selection 



Alkali digestion value


Disease bacterial blight


Brown planthopper


Culm length


Days to heading


Grain weight (GW)


Marker-assisted selection


Number of filled grains per panicle


Number of panicles


Panicle length


Recurrent parent


Rice grassy stunt virus


Rice ragged stunt virus


Rice stripe virus


Seed set rate


Xanthomonas oryzae pv. oryzae



This research was based on germplasm developed by Dr. K.K. Jena and was supported in part by a grant to the Agenda Program (Code No. PJ00926202), the Temperate Rice Research Consortium (TRRC) of the Rural Development Administration, Republic of Korea, and the Global Rice Science Partnership (GRiSP) program of IRRI. We thank the IRRI communication team for carefully editing the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest.

Ethical standards

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

438_2018_1470_MOESM1_ESM.docx (38 kb)
Supplementary material 1 (DOCX 37 KB)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Strategic Innovation PlatformInternational Rice Research InstituteMetro ManilaPhilippines
  2. 2.C/o IRRI-Korea Office, National Institute of Crop Science, Rural Development AdministrationIseo-myeon, Wanju-gunRepublic of Korea
  3. 3.National Institute of Crop Science, Rural Development AdministrationIseo-myeon, Wanju-gunRepublic of Korea

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