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Molecular Genetics and Genomics

, Volume 291, Issue 4, pp 1625–1638 | Cite as

Detection and validation of one stable fiber strength QTL on c9 in tetraploid cotton

  • X. Yang
  • Y. Wang
  • G. Zhang
  • X. Wang
  • L. Wu
  • H. Ke
  • H. Liu
  • Z. MaEmail author
Original Article

Abstract

Fiber strength is an essential trait of fiber property in cotton, and it is quantitatively inherited. Identification of stable quantitative trait loci (QTL) contributing to fiber strength would provide the key basis for marker-assisted selection (MAS) in cotton breeding. In this study, four interspecific hybridization populations were established with a common G. barbadense parent Pima 90-53 and two G. hirsutum parents (CCRI 8 and Handan 208), each of which had fiber strength characteristic. Based on the phenotypic data of fiber strength from seven environments, a stable QTL, qFS-c9-1, was detected and validated on c9 in a marker interval between SSR markers NAU2395 and NAU1092. The QTL explaining 14.4–17.9 % of the phenotypic variation was firstly detected in two populations (CCRI 8 × Pima 90-53, BC1F1 and BC1F2) and its derived lines in four environments. And it accounting for 12.1–14.8 % of the phenotypic variation was further confirmed in two populations (Handan 208 × Pima 90-53, BC1F1, and F2) under one environment. In silico mapping using three sequenced cotton genomes indicated that homologous genes, anchored by NAU2395 and NAU1092, were aligned to the G. arboreum genome within a physical distance between 81.10 Mbps and 87.07 Mbps. In that interval, several genes were confirmed in literatures to associate with fiber development. Among these genes, seven genes were further selected for an expression analysis through fiber development transcriptome database, revealing unique expression patterns across different stages of fiber development between CCRI 8 and Pima 90-53. The genes underlying qFS-c9-1 were favorable to fine mapping and cloning. The current study results provided valuable evidence for mapping stable QTL of fiber strength utilizing multiple populations and environments, as well as map-based cloning the candidate gene underlying the QTL for future prospective research directions.

Keywords

Cotton Fiber strength Simple sequence repeat (SSR) QTL 

Notes

Acknowledgments

This study was funded by the National High Technology Research and Development Program of China (Grant Number 2012AA101108-02) and a project from Ministry of Agriculture of China for Transgenic Research (Grant Number 2014ZX08009-003).

Compliance with ethical standards

Conflict of interest

Authors declare that they have no conflict of interest.

Supplementary material

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Supplementary material 1 (DOCX 401 kb)
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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • X. Yang
    • 1
  • Y. Wang
    • 1
  • G. Zhang
    • 1
  • X. Wang
    • 1
  • L. Wu
    • 1
  • H. Ke
    • 1
  • H. Liu
    • 1
  • Z. Ma
    • 1
    Email author
  1. 1.North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Key Laboratory of Crop Germplasm Resources of HebeiHebei Agricultural UniversityBaodingPeople’s Republic of China

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