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Molecular Genetics and Genomics

, Volume 291, Issue 4, pp 1523–1534 | Cite as

Fine mapping and candidate gene analysis of an anthocyanin-rich gene, BnaA.PL1, conferring purple leaves in Brassica napus L.

  • Haibo Li
  • Lixia Zhu
  • Gaigai Yuan
  • Shuangping Heng
  • Bin Yi
  • Chaozhi Ma
  • Jinxiong Shen
  • Jinxing Tu
  • Tingdong Fu
  • Jing WenEmail author
Original Article

Abstract

Because of the advantages of anthocyanins, the genetics and breeding of crops rich in anthocyanins has become a hot research topic. However, due to the lack of anthocyanin-related mutants, no regulatory genes have been mapped in Brassica napus. In this study, we first report the characterization of a B. napus line with purple leaves and the fine mapping and candidate screening of the BnaA.PL1 gene. The amount of anthocyanins in the purple leaf line was six times higher than that in a green leaf line. A genetic analysis indicated that the purple character was controlled by an incomplete dominant gene. Through map-based cloning, we localized the BnaA.PL1 gene to a 99-kb region at the end of B. napus chromosome A03. Transcriptional analysis of 11 genes located in the target region revealed that the expression level of only the BnAPR2 gene in seedling leaves decreased from purple to reddish green to green individuals, a finding that was consistent with the measured anthocyanin accumulation levels. Molecular cloning and sequence analysis of BnAPR2 showed that the purple individual-derived allele contained 17 variants. Markers co-segregating with BnaA.PL1 were developed from the sequence of BnAPR2 and were validated in the BC4P2 population. These results suggested that BnAPR2, which encodes adenosine 5′-phosphosulfate reductase, is likely to be a valuable candidate gene. This work may lay the foundation for the marker-assisted selection of B. napus vegetables that are rich in anthocyanins and for an improved understanding of the molecular mechanisms controlling anthocyanin accumulation in Brassica.

Keywords

Anthocyanin-rich mutant Brassica napus Fine mapping Purple leaf trait 

Notes

Acknowledgments

This research was financed by the Program of Introducing Talents of Discipline to Universities in China (the 111 Project No. B14032), the Program for Modern Agricultural Industrial Technology System (nycytx-00501), the National Support Program (2011BAD35B04), the Hi-Tech Research and Development Program of China (2011AA10A104), and the Science and Technology Projects of Shaoguan (2013CXY/C13, 2014CX/N323).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

438_2016_1199_MOESM1_ESM.doc (694 kb)
Supplementary material 1 (DOC 694 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Haibo Li
    • 1
    • 2
  • Lixia Zhu
    • 1
  • Gaigai Yuan
    • 1
  • Shuangping Heng
    • 1
  • Bin Yi
    • 1
  • Chaozhi Ma
    • 1
  • Jinxiong Shen
    • 1
  • Jinxing Tu
    • 1
  • Tingdong Fu
    • 1
  • Jing Wen
    • 1
    Email author
  1. 1.National Key Laboratory of Crop Genetic Improvement, National Sub-center of Rapeseed Improvement in WuhanHuazhong Agricultural UniversityWuhanChina
  2. 2.College of Yingdong Agricultural Science and EngineeringShaoguan UniversityShaoguanChina

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