Molecular Genetics and Genomics

, Volume 290, Issue 5, pp 1639–1657 | Cite as

miRNAome analysis associated with anatomic and transcriptomic investigations reveal the polar exhibition of corky split vein in boron deficient Citrus sinensis

  • Chengquan Yang
  • Tao Liu
  • Fuxi Bai
  • Nannan Wang
  • Zhiyong Pan
  • Xiang Yan
  • ShuAng PengEmail author
Original Paper


Corky split vein can develop under long-term boron deficient conditions in Citrus sinensis L. Osbeck cv. Newhall. This symptom only occurs in the upper rather than the lower epidermis of old leaves. Our previous study demonstrated that vascular hypertrophy was involved in the symptoms, and the 3rd developmental stage of corky split vein (BD3) was the critical stage for phenotype formation. Here, we performed an intensive study on the BD3 vein and its control sample (CK3 vein). A lignin test demonstrated that the lignin content in BD3 vein was approximately 1.7 times more than the CK3 vein. Anatomical investigation of the corky split vein indicated that the upper epidermis was destroyed by overgrowing vascular cells, and the increased lignin may contribute to vascular cell differentiation and wounding-induced lignification. In a subsequent small RNA sequencing of the BD3 and CK3 veins, 99 known miRNAs and 22 novel miRNAs were identified. Comparative profiling of these miRNAs demonstrated that the 57 known miRNAs and all novel miRNAs exhibited significant expression differences between the two small RNAs libraries of the BD3 and CK3 veins. Associated with our corresponding digital gene expression data, we propose that the decreased expression of two miRNAs, csi-miR156b and csi-miR164, which leads to the up-regulation of their target genes, SPLs (csi-miR156b-targeted) and CUC2 (csi-miR164-targeted), may promote vascular cell division and orderless stage transition in old leaves.


Corky split vein Anatomy miRNAome Lignification Cell division Orderless stage transition 



This research was financially supported by the National Modern Citrus Industry System, the Ministry of Education Innovation Team (IRT13065), the National NSF of China (No. 31071761 and 31272121).

Supplementary material

438_2015_1024_MOESM1_ESM.xlsx (12 kb)
Supplementary Table S1 Primer sequences of the miRNAs and their targets for qRT-PCR analysis (XLSX 11 kb)
438_2015_1024_MOESM2_ESM.xlsx (5.4 mb)
Supplementary Table S2 Known miRNAs in the C. sinensis vein (XLSX 5490 kb)
438_2015_1024_MOESM3_ESM.xlsx (11 kb)
Supplementary Table S3 Novel miRNAs in the C. sinensis vein (XLSX 10 kb)
438_2015_1024_MOESM4_ESM.xlsx (2.7 mb)
Supplementary Table S4 Differentially expressed known miRNA list (XLSX 2748 kb)
438_2015_1024_MOESM5_ESM.xlsx (10 kb)
Supplementary Table S5 Differentially expressed novel miRNA list (XLSX 10 kb)
438_2015_1024_MOESM6_ESM.xlsx (524 kb)
Supplementary Table S6 Differentially expressed gene list (XLSX 523 kb)
438_2015_1024_MOESM7_ESM.xlsx (14 kb)
Supplementary Table S7 Predicted target genes of differentially expressed known miRNAs (XLSX 13 kb)
438_2015_1024_MOESM8_ESM.xlsx (12 kb)
Supplementary Table S8 Predicted target genes of novel miRNAs (XLSX 12 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Chengquan Yang
    • 1
    • 2
    • 3
  • Tao Liu
    • 1
    • 2
    • 3
  • Fuxi Bai
    • 1
    • 2
    • 3
  • Nannan Wang
    • 1
    • 2
    • 3
  • Zhiyong Pan
    • 1
    • 2
    • 3
  • Xiang Yan
    • 1
    • 2
    • 3
  • ShuAng Peng
    • 1
    • 2
    • 3
    Email author
  1. 1.College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhanChina
  2. 2.Key Laboratory of Horticultural Plant BiologyMinistry of EducationWuhanChina
  3. 3.Key Laboratory of Horticultural Crop Biology and Genetic Improvement (Central Region)MOAWuhanChina

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