Molecular Genetics and Genomics

, Volume 289, Issue 3, pp 399–409

A novel tomato mutant, Solanum lycopersicumelongated fruit1 (Slelf1), exhibits an elongated fruit shape caused by increased cell layers in the proximal region of the ovary

  • Katarut Chusreeaeom
  • Tohru Ariizumi
  • Erika Asamizu
  • Yoshihiro Okabe
  • Kenta Shirasawa
  • Hiroshi Ezura
Original Paper

DOI: 10.1007/s00438-014-0822-8

Cite this article as:
Chusreeaeom, K., Ariizumi, T., Asamizu, E. et al. Mol Genet Genomics (2014) 289: 399. doi:10.1007/s00438-014-0822-8

Abstract

Genes controlling fruit morphology offer important insights into patterns and mechanisms determining organ shape and size. In cultivated tomato (Solanum lycopersicum L.), a variety of fruit shapes are displayed, including round-, bell pepper-, pear-, and elongate-shaped forms. In this study, we characterized a tomato mutant possessing elongated fruit morphology by histologically analyzing its fruit structure and genetically analyzing and mapping the genetic locus. The mutant line, Solanum lycopersicumelongated fruit 1 (Slelf1), was selected in a previous study from an ethylmethane sulfonate-mutagenized population generated in the background of Micro-Tom, a dwarf and rapid-growth variety. Histological analysis of the Slelf1 mutant revealed dramatically increased elongation of ovary and fruit. Until 6 days before flowering, ovaries were round and they began to elongate afterward. We also determined pericarp thickness and the number of cell layers in three designated fruit regions. We found that mesocarp thickness, as well as the number of cell layers, was increased in the proximal region of immature green fruits, making this the key sector of fruit elongation. Using 262 F2 individuals derived from a cross between Slelf1 and the cultivar Ailsa Craig, we constructed a genetic map, simple sequence repeat (SSR), cleaved amplified polymorphism sequence (CAPS), and derived CAPS (dCAPS) markers and mapped to the 12 tomato chromosomes. Genetic mapping placed the candidate gene locus within a 0.2 Mbp interval on the long arm of chromosome 8 and was likely different from previously known loci affecting fruit shape.

Keywords

Tomato (Solanum lycopersicumMutant Genetic mapping Fruit shape Cell division 

Abbreviations

AC

Ailsa Craig

CAPS

Cleaved amplified polymorphic sequences

DAF

Days after flowering

DAP

Days after planting

DBF

Days before flowering

dCAPs

Derived cleaved amplified polymorphic sequence

EMS

Ethylmethane sulfonate

NBRP

National BioResource Project

Slelf1

Solanum lycopersicumelongated fruit 1

SSR markers

Simple sequence repeat markers

TES markers

Tomato expressed sequence tag (EST)-derived SSRmarkers

TGS marker

Tomato genome-derived SSR markers

WT

Wild type

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Katarut Chusreeaeom
    • 1
  • Tohru Ariizumi
    • 1
  • Erika Asamizu
    • 2
  • Yoshihiro Okabe
    • 1
  • Kenta Shirasawa
    • 2
  • Hiroshi Ezura
    • 1
  1. 1.Graduate School of Life and Environmental SciencesUniversity of TsukubaTsukubaJapan
  2. 2.Kazusa DNA Research InstituteKisarazuJapan

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