Molecular Genetics and Genomics

, Volume 289, Issue 3, pp 399–409 | Cite as

A novel tomato mutant, Solanum lycopersicum elongated fruit1 (Slelf1), exhibits an elongated fruit shape caused by increased cell layers in the proximal region of the ovary

  • Katarut Chusreeaeom
  • Tohru Ariizumi
  • Erika Asamizu
  • Yoshihiro Okabe
  • Kenta Shirasawa
  • Hiroshi Ezura
Original Paper


Genes controlling fruit morphology offer important insights into patterns and mechanisms determining organ shape and size. In cultivated tomato (Solanum lycopersicum L.), a variety of fruit shapes are displayed, including round-, bell pepper-, pear-, and elongate-shaped forms. In this study, we characterized a tomato mutant possessing elongated fruit morphology by histologically analyzing its fruit structure and genetically analyzing and mapping the genetic locus. The mutant line, Solanum lycopersicum elongated fruit 1 (Slelf1), was selected in a previous study from an ethylmethane sulfonate-mutagenized population generated in the background of Micro-Tom, a dwarf and rapid-growth variety. Histological analysis of the Slelf1 mutant revealed dramatically increased elongation of ovary and fruit. Until 6 days before flowering, ovaries were round and they began to elongate afterward. We also determined pericarp thickness and the number of cell layers in three designated fruit regions. We found that mesocarp thickness, as well as the number of cell layers, was increased in the proximal region of immature green fruits, making this the key sector of fruit elongation. Using 262 F2 individuals derived from a cross between Slelf1 and the cultivar Ailsa Craig, we constructed a genetic map, simple sequence repeat (SSR), cleaved amplified polymorphism sequence (CAPS), and derived CAPS (dCAPS) markers and mapped to the 12 tomato chromosomes. Genetic mapping placed the candidate gene locus within a 0.2 Mbp interval on the long arm of chromosome 8 and was likely different from previously known loci affecting fruit shape.


Tomato (Solanum lycopersicumMutant Genetic mapping Fruit shape Cell division 



Ailsa Craig


Cleaved amplified polymorphic sequences


Days after flowering


Days after planting


Days before flowering


Derived cleaved amplified polymorphic sequence


Ethylmethane sulfonate


National BioResource Project


Solanum lycopersicum elongated fruit 1

SSR markers

Simple sequence repeat markers

TES markers

Tomato expressed sequence tag (EST)-derived SSRmarkers

TGS marker

Tomato genome-derived SSR markers


Wild type



The authors thank the National BioResearch Project (NBRP), MEXT, Japan, for providing the seed stocks. We also thank all members of Prof. Hiroshi Ezura’s laboratory for helpful discussions throughout the work.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Katarut Chusreeaeom
    • 1
  • Tohru Ariizumi
    • 1
  • Erika Asamizu
    • 2
  • Yoshihiro Okabe
    • 1
  • Kenta Shirasawa
    • 2
  • Hiroshi Ezura
    • 1
  1. 1.Graduate School of Life and Environmental SciencesUniversity of TsukubaTsukubaJapan
  2. 2.Kazusa DNA Research InstituteKisarazuJapan

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