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Molecular Genetics and Genomics

, Volume 288, Issue 11, pp 591–599 | Cite as

Silencing of TaBTF3 gene impairs tolerance to freezing and drought stresses in wheat

  • Guozhang Kang
  • Hongzhen Ma
  • Guoqin Liu
  • Qiaoxia Han
  • Chengwei Li
  • Tiancai Guo
Original Paper

Abstract

Basic transcription factor 3 (BTF3), the β-subunit of the nascent polypeptide-associated complex, is responsible for the transcriptional initiation of RNA polymerase II and is also involved in cell apoptosis, translation initiation regulation, growth, development, and other functions. Here, we report the impact of BTF3 on abiotic tolerance in higher plants. The transcription levels of the TaBTF3 gene, first isolated from wheat seedlings in our lab, were differentially regulated by diverse abiotic stresses and hormone treatments, including PEG-induced stress (20 % polyethylene glycol 6000), cold (4 °C), salt (100 mM NaCl), abscisic acid (100 μM), methyl jasmonate (50 μM), and salicylic acid (50 μM). Southern blot analysis indicated that, in the wheat genome, TaBTF3 is a multi-copy gene. Compared to BSMV-GFP-infected wheat plants (control), under freezing (−8 °C for 48 h) or drought stress (withholding water for 15 days) conditions, TaBTF3-silenced wheat plants showed lower survival rates, free proline content, and relative water content and higher relative electrical conductivity and water loss rate. These results suggest that silencing of the TaBTF3 gene may impair tolerance to freezing and drought stresses in wheat and that it may be involved in the response to abiotic stresses in higher plants.

Keywords

Drought Freezing Gene silencing TaBTF3 Triticum aestivum L. 

Notes

Acknowledgments

We sincerely thank Prof. Daowen Wang for kindly providing the BSMV vectors (Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing). This work was supported by the Special Modern Agricultural Industry (Wheat) Technology System (CARS-03), the National Natural Science Foundation of China (31171471), and the Open Item of the State Key Laboratory of Crop Biology (2013KF04).

Conflict of interest

The authors declare no conflict of interest.

References

  1. Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–207CrossRefGoogle Scholar
  2. Bloss TA, Wilze ES, Rothman JH (2003) Suppression of CED-3-independent apoptosis by mitochondrial NAC in Caenorhabditis elegans. Nature 424:1066–1071PubMedCrossRefGoogle Scholar
  3. Diallo A, Kane N, Agharbaoui Z, Badawi M, Sarhan F (2010) Heterologous expression of wheat VERNALIZATION 2 (TaVRN2) gene in Arabidopsis delays flowering and enhances freezing tolerance. PlosOne 5:e8690CrossRefGoogle Scholar
  4. Freire MA (2005) Translation initiation factor (iso) 4E interacts with BTF3, the beta subunit of the nascent polypeptide-associated complex. Gene 345:271–277PubMedCrossRefGoogle Scholar
  5. Frizzi A, Huang S (2010) Tapping RNA silencing pathways for plant biotechnology. Plant Biotech J 8:655–677CrossRefGoogle Scholar
  6. Gao SQ, Chen M, Xia LQ, Xiu HJ, Xu ZS, Li LC, Zhao CP, Cheng XG, Ma YZ (2009) A cotton (Gossypium hirsutum) DRE-binding transcription factor gene, GhDREB, confers enhanced tolerance to drought, high salt, and freezing stresses in transgenic wheat. Plant Cell Rep 28:301–311PubMedCrossRefGoogle Scholar
  7. Gasparis S, Orczyk W, Zalewski W, Nadolska-Orczyk A (2011) The RNA-mediated silencing of one of the Pin genes in allohexaploid wheat simultaneously decreases the expression of the other, and increases grain hardness. J Exp Bot 62:4025–4036PubMedCrossRefGoogle Scholar
  8. Grill E, Himmelbach A (1998) ABA signal transduction. Curr Opin Plant Biol 5:412–418CrossRefGoogle Scholar
  9. Hein I, Barciszewska-Pacak M, Hrubikova K, Williamson S, Dinesen M, Soenderby IE, Sundar S, Jarmolowske A, Shirasu K, Lacomme C (2005) Virus-induced gene silencing-based functional characterization of genes associated with powdery mildew resistance in barley. Plant Physiol 138:2155–2164PubMedCrossRefGoogle Scholar
  10. Herman EM, Rotter K, Premakumar R, Elwinger G, Bae R, Ehler-King L, Chen S, Livingston DP (2006) Additional freeze hardiness in wheat acquired by exposure to −3 °C is associated with extensive physiological, morphological, and molecular changes. J Exp Bot 57:3601–3618PubMedCrossRefGoogle Scholar
  11. Horváth E, Szalai G, Janda T (2007) Induction of abiotic stress tolerance by salicylic acid signaling. J Plant Growth Regul 26:290–300CrossRefGoogle Scholar
  12. Huh SU, Kim KJ, Paek KH (2012) Capsicum annuum basic transcription factor 3 (CaBTF3) regulates transcription of pathogenesis-related genes during hypersensitive response upon tobacco mosaic virus infection. Biochem Bioph Res Co 417:910–917CrossRefGoogle Scholar
  13. Karan R, Subudhi PK (2012) Overexpression of a nascent polypeptide associated complex gene (SaβNAC) of Spartina alterniflora improves tolerance to salinity and drought in transgenic Arabidopsis. Biochem Bioph Res Co 424:747–752CrossRefGoogle Scholar
  14. Liang JJ, Deng GB, Long H, Pan ZF, Wang CP, Cai P, Xu DL, Nima ZX, Yu MQ (2012) Virus-induced silencing of genes encoding LEA protein in Tibetan hulless barley (Hordeum vulgare ssp. vulgare) and their relationship to drought tolerance. Mol Breed 30:441–451CrossRefGoogle Scholar
  15. Ma HZ, Liu GQ, Li CW, Kang GZ, Guo TC (2012) Identification of the TaBTF3 gene in wheat (Triticum aestivum L.) and the effect of its silencing on wheat chloroplast, mitochondria and mesophyll cell development. Biochem Bioph Res Co 426:608–614CrossRefGoogle Scholar
  16. Mao XG, Zhang HY, Qian XY, Li A, Zhao GY, Jing RL (2012) TaNAC2, a NAC-type wheat transcription factor conferring enhanced multiple abiotic stress tolerances in Arabidopsis. J Exp Bot 63:2933–2964PubMedCrossRefGoogle Scholar
  17. Pei ZF, Ming DF, Liu D, Wan GL, Geng XX, Gong HJ, Zhou WJ (2010) Silicon improves the tolerance to water-deficit stress induced by polyethylene glycol in wheat (Triticum aestivum L.) seedlings. J Plant Growth Regul 29:106–115CrossRefGoogle Scholar
  18. Purkayastha A, Dasgupta I (2009) Virus-induced gene silencing: a versatile tool for discovery of gene functions in plants. Plant Physiol Biochem 47:967–976PubMedCrossRefGoogle Scholar
  19. Rospert S, Dubaquie Y, Gautschi M (2002) Nascent-polypeptide-associated complex. Cell Mol Life Sci 59:1632–1639PubMedCrossRefGoogle Scholar
  20. Sahu GK, Kar M, Sabat SC (2010) Alteration in phosphate uptake potential of wheat plants co-cultivated with salicylic acid. J Plant Physiol 167:326–328PubMedCrossRefGoogle Scholar
  21. Seckin B, Sekmen AH, Türkan I (2009) An enhancing effect of exogenous mannitol on the antioxidant enzyme activities in roots of wheat under salt stress. J Plant Growth Regul 28:12–20CrossRefGoogle Scholar
  22. Singh HP, Batish DR, Kohli RK, Arora K (2007) Arsenic-induced root growth inhibition in mung bean (Phaseolus aureus Roxb.) is due to oxidative stress resulting from enhanced lipid peroxidation. Plant Growth Regul 53:65–73CrossRefGoogle Scholar
  23. Song S, Chen Y, Zhao M, Zhang WH (2012) A novel Medicago truncatula HD-Zip gene, MtHB2, is involved in abiotic stress responses. Environ Exp Bot 80:1–9CrossRefGoogle Scholar
  24. Tufan HA, Stefanato FL, McGrann GRD, MacCormack R, Boyd LA (2011) The Barley stripe mosaic virus system used for virus-induced gene silencing in cereals differentially affects susceptibility to fungal pathogens in wheat. J Plant Physiol 168:990–994PubMedCrossRefGoogle Scholar
  25. Wasternack C, Hause B (2002) Jasmonates and octadecanoids: signals in plant stress responses and development. Prog Nucleic Acid Res Mol Biol 72:165–221PubMedCrossRefGoogle Scholar
  26. Wiedmann B, Sakai H, Davis TA, Wiedmann M (1994) A protein complex required for signal-sequence-specific sorting and translocation. Nature 370:434–440PubMedCrossRefGoogle Scholar
  27. Withers LA, King PJ (1979) Proline: a novel cryoprotectant for the freeze preservation of cultured cells of Zea mays L. Plant Physiol 64:675–678PubMedCrossRefGoogle Scholar
  28. Wu H, Sparks C, Amoah B, Jones HD (2003) Factors influencing successful Agrobacterium- mediated genetic transformation of wheat. Plant Cell Rep 21:659–666PubMedGoogle Scholar
  29. Yang KS, Kim HS, Jin UH, Lee SS, Park JA, Lim YP, Pai HS (2007) Silencing of NbBTF3 results in developmental defects and disturbed gene expression in chloroplasts and mitochondria of higher plants. Planta 225:1459–1469PubMedCrossRefGoogle Scholar
  30. Zhao T, Zhao S, Chen H, Zhao Q, Hu Z, Hou B, Xia G (2006) Transgenic wheat progeny resistant to powdery mildew generated by Agrobacterium inoculum to the basal portion of wheat seedling. Plant Cell Rep 25:1199–1204PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Guozhang Kang
    • 1
  • Hongzhen Ma
    • 1
  • Guoqin Liu
    • 1
  • Qiaoxia Han
    • 1
  • Chengwei Li
    • 2
  • Tiancai Guo
    • 1
  1. 1.National Engineering Research Centre for Wheat, The Key Laboratory of Physiology, Ecology and Genetic Improvement of Food Crops in Henan ProvinceHenan Agricultural UniversityZhengzhouChina
  2. 2.The Collaborative Innovation Center of Henan Food CropsHenan Agricultural UniversityZhengzhouChina

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