An active hAT transposable element causing bud mutation of carnation by insertion into the flavonoid 3′-hydroxylase gene
The molecular mechanisms underlying spontaneous bud mutations, which provide an important breeding tool in carnation, are poorly understood. Here we describe a new active hAT type transposable element, designated Tdic101, the movement of which caused a bud mutation in carnation that led to a change of flower color from purple to deep pink. The color change was attributed to Tdic101 insertion into the second intron of F3′H, the gene for flavonoid 3′-hydroxylase responsible for purple pigment production. Regions on the deep pink flowers of the mutant can revert to purple, a visible phenotype of, as we show, excision of the transposable element. Sequence analysis revealed that Tdic101 has the characteristics of an autonomous element encoding a transposase. A related, but non-autonomous element dTdic102 was found to move in the genome of the bud mutant as well. Its mobilization might be the result of transposase activities provided by other elements such as Tdic101. In carnation, therefore, the movement of transposable elements plays an important role in the emergence of a bud mutation.
KeywordshAT Active transposable element Bud mutation Flavonoid 3′-hydroxylase Dianthus
We thank to Takayasu Hirosawa, Hiroshi Okawa and Noboru Onishi for helpful discussions. This work was partly supported by a grant from the “Technical Development Program for Making Agribusiness in the Form of Utilizing the Concentrated Know-how from the Private Sector” of the Ministry of Agriculture, Forestry and Fisheries JAPAN.
- Abe Y, Tera M, Sasaki N, Okamura M, Umemoto N, Momose M, Kawahara N, Kamakura H, Goda Y, Nagasawa K, Ozeki Y (2008) Detection of 1-O-malylglucose: pelargonidin 3-O-glucose-6′′-O-malyltransferase activity in carnation (Dianthus caryophyllus). Biochem Biophys Res Commun 373:473–477PubMedCrossRefGoogle Scholar
- Geissman TA, Mehlquist GA (1947) Inheritance in the carnation, Dianthus caryophyllus: the chemistry of flower color variation. Genetics 32:410–433Google Scholar
- Inagaki Y, Hisatomi Y, Iida S (1996) Somatic mutations caused by excision of the transposable element, Tpn1, from the DFR gene for pigmentation in sub-epidermal layer of periclinally chimeric flowers of Japanese morning glory and their germinal transmission to their progeny. Theoret Appl Genet 92:499–504CrossRefGoogle Scholar
- Itoh Y, Higeta D, Suzuki A, Yoshida H, Ozeki Y (2002) Excision of transposable elements from the chalcone isomerase and dihydroflavonol 4-reductase genes may contribute to the variegation of the yellow-flowered carnation (Dianthus caryophyllus). Plant Cell Physiol 43:578–585PubMedCrossRefGoogle Scholar
- Matsuba Y, Sasaki N, Tera M, Okamura M, Abe Y, Okamoto E, Nakamura H, Funabashi H, Takatsu M, Saito M, Matsuoka H, Nagasawa K, Ozeki Y (2010) A novel glucosylation reaction on anthocyanins catalyzed by acyl-glucose-dependent glucosyltransferase in the petals of carnation and delphinium. Plant Cell 22:3374–3389PubMedCrossRefGoogle Scholar
- Shibata M, Kishimoto S, Hirai M, Aida R, Ikeda I (1998) Analysis of the periclinal chimeric structure of chrysanthemum sports by random amplified polymorphic DNA. Acta Hortic 454:347–353Google Scholar
- Ueyama Y, Suzuki K-i, Fukuchi-Mizutani M, Fukui Y, Miyazaki K, Ohkawa H, Kusumi T, Tanaka Y (2002) Molecular and biochemical characterization of torenia flavonoid 3′-hydroxylase and flavone synthase II and modification of flower color by modulating the expression of these genes. Plant Sci 163:253–263CrossRefGoogle Scholar
- Yakushiji H, Kobayashi S, Goto-Yamamoto N, Tae Jeong S, Sueta T, Mitani N, Azuma A (2006) A skin color mutation of grapevine, from black-skinned pinot noir to white-skinned pinot blanc, is caused by deletion of the functional VvmybA1 allele. Biosci Biotechnol Biochem 70:1506–1508PubMedCrossRefGoogle Scholar