Whole-genome sequencing of the efficient industrial fuel-ethanol fermentative Saccharomyces cerevisiae strain CAT-1
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The Saccharomyces cerevisiae strains widely used for industrial fuel-ethanol production have been developed by selection, but their underlying beneficial genetic polymorphisms remain unknown. Here, we report the draft whole-genome sequence of the S. cerevisiae strain CAT-1, which is a dominant fuel-ethanol fermentative strain from the sugarcane industry in Brazil. Our results indicate that strain CAT-1 is a highly heterozygous diploid yeast strain, and the ~12-Mb genome of CAT-1, when compared with the reference S228c genome, contains ~36,000 homozygous and ~30,000 heterozygous single nucleotide polymorphisms, exhibiting an uneven distribution among chromosomes due to large genomic regions of loss of heterozygosity (LOH). In total, 58 % of the 6,652 predicted protein-coding genes of the CAT-1 genome constitute different alleles when compared with the genes present in the reference S288c genome. The CAT-1 genome contains a reduced number of transposable elements, as well as several gene deletions and duplications, especially at telomeric regions, some correlated with several of the physiological characteristics of this industrial fuel-ethanol strain. Phylogenetic analyses revealed that some genes were likely associated with traits important for bioethanol production. Identifying and characterizing the allelic variations controlling traits relevant to industrial fermentation should provide the basis for a forward genetics approach for developing better fermenting yeast strains.
KeywordsBioethanol Genome Saccharomyces Sugarcane Industrial strains
The work of B.U.S. at Stanford University was possible through a visiting fellowship (BEX2793-05-9) from CAPES, Brazil.
- Andrietta MGS, Andrietta SR, Steckelberg C, Stupiello ENA (2007) Bioethanol—Brazil, 30 years of Proálcool. Int Sugar J 109:195–200Google Scholar
- Duval EH, Alves-Jr SL, Dunn B, Sherlock G, Stambuk BU (2010) Microarray karyotyping of maltose-fermenting Saccharomyces yeasts with differing maltotriose utilization profiles reveals copy number variation in genes involved in maltose and maltotriose utilization. J Appl Microbiol 109:248–259PubMedGoogle Scholar
- Felsenstein J (1989) Phylogeny Inference Package (Version 3.2). Cladistics 5:164–166Google Scholar
- Leal MRLV, Walter AD (2010) Sustainability of the production of ethanol from sugarcane: the Brazilian experience. Int Sugar J 112:390–396Google Scholar
- Novo M, Bigey F, Beyne E, Galeote V, Gavory F, Mallet S, Cambon B, Legras JL, Wincker P, Casaregola S, Dequin S (2009) Eukaryote-to-eukaryote gene transfer events revealed by the genome sequence of the wine yeast Saccharomyces cerevisiae EC1118. Proc Natl Acad Sci USA 106:16333–16338PubMedCrossRefGoogle Scholar
- Park JI, Grant CM, Dawes IW (2005) The high-affinity cAMP phosphodiesterase of Saccharomyces cerevisiae is the major determinant of cAMP levels in stationary phase: involvement of different branches of the Ras-cyclic AMP pathway in stress responses. Biochem Biophys Res Commun 327:311–319PubMedCrossRefGoogle Scholar
- Rose MD, Winston F, Hieter P (1990) Methods in yeast genetics. Cold Spring Harbor Laboratory Press, New YorkGoogle Scholar