Molecular Genetics and Genomics

, Volume 285, Issue 2, pp 125–149 | Cite as

Genome-wide screen for inositol auxotrophy in Saccharomyces cerevisiae implicates lipid metabolism in stress response signaling

  • Manuel J. Villa-García
  • Myung Sun Choi
  • Flora I. Hinz
  • María L. Gaspar
  • Stephen A. Jesch
  • Susan A. Henry
Original Paper

Abstract

Inositol auxotrophy (Ino phenotype) in budding yeast has classically been associated with misregulation of INO1 and other genes involved in lipid metabolism. To identify all non-essential yeast genes that are necessary for growth in the absence of inositol, we carried out a genome-wide phenotypic screening for deletion mutants exhibiting Ino phenotypes under one or more growth conditions. We report the identification of 419 genes, including 385 genes not previously reported, which exhibit this phenotype when deleted. The identified genes are involved in a wide range of cellular processes, but are particularly enriched in those affecting transcription, protein modification, membrane trafficking, diverse stress responses, and lipid metabolism. Among the Ino mutants involved in stress response, many exhibited phenotypes that are strengthened at elevated temperature and/or when choline is present in the medium. The role of inositol in regulation of lipid metabolism and stress response signaling is discussed.

Keywords

Yeast Inositol auxotrophy Inositol Lipid metabolism Stress response 

Abbreviations

Ino

Inositol auxotrophy

RNA-Pol II

RNA polymerase II

PI

Phosphatidylinositol

PA

Phosphatidic acid

ER

Endoplasmic reticulum

DAG

Diacylglycerol

PC

Phosphatidylcholine

PKC–CWI

Protein kinase C–cell wall integrity

UPR

Unfolded protein response

AMPK

AMP-dependant kinase

HOG

High osmolarity glycerol

ERAD

ER-associated protein degradation

TOR

Target of rapamycin

PKA

cAMP-protein kinase A

MAPK

Mitogen activated protein kinase

ORF

Open reading frame

GO

Gene Ontology

SGD

Saccharomyces genome database

YPD

1% yeast extract, 2% bactopeptone, 2% glucose

I

Inositol

C

Choline

GroPCho

Glycerol-phospho-choline

GPI

Glycosylphosphatidylinositol

Gro-3-P

Glycerol-3-phosphate

lyso-PA

Lyso-phosphatidic acid

DHA

Dihydroxyacetone

DHAP

Dihydroxyacetone phosphate

PIP

Phosphoinositides

IP3

Inositol 1,4,5-triphosphate

IPC

Inositol-phosphorylceramide

MIPC

Mannosyl-inositol-phosphorylceramide

M(IP)2C

Mannosyl-diinositol-phosphorylceramide

Notes

Ackowledgments

This work was supported by National Institutes of Health Grant GM019629 (to SAH.)

Supplementary material

438_2010_592_MOESM1_ESM.pdf (122 kb)
Supplementary material 1 (PDF 122 kb)
438_2010_592_MOESM2_ESM.pdf (299 kb)
Supplementary material 2 (PDF 299 kb)
438_2010_592_MOESM3_ESM.xls (101 kb)
Supplementary material 3 (XLS 101 kb)

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Manuel J. Villa-García
    • 1
  • Myung Sun Choi
    • 1
  • Flora I. Hinz
    • 1
  • María L. Gaspar
    • 1
  • Stephen A. Jesch
    • 1
  • Susan A. Henry
    • 1
  1. 1.Department of Molecular Biology and GeneticsCornell UniversityIthacaUSA

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