Many ways to generate microRNA-like small RNAs: non-canonical pathways for microRNA production
- 1.6k Downloads
MicroRNAs (miRNAs) are an abundant class of small non-coding RNAs that collectively regulate the expression of a large number of mRNAs by either promoting destabilization or repressing translation, or both. Therefore, they play a major role in shaping the transcriptomes and proteomes of eukaryotic organisms. Typically, animal miRNAs are produced from long primary transcripts with one or more of hairpin structures by two sequential processing reactions: one by Drosha in the nucleus and the other by Dicer in the cytoplasm. However, deviations from this paradigm have been observed: subclasses of miRNAs, which only partially meet the classical definition of a miRNA, are derived by alternative biogenesis pathways, thereby providing an additional level of complexity to miRNA-dependent regulation of gene expression.
KeywordsmiRNA Drosha Dicer Argonaute snoRNA tRNA
The authors would like to thank the members of the Siomi Laboratory for discussions. This work was supported by MEXT grants to H.S and K.M, and a Keio University Grant-in-Aid for Encouragement of Young Medical Scientists to K.M.
- Berezikov E, van Tetering G, Verheul M, van de Belt J, van Laake L, Vos J, Verloop R, van de Wetering M, Guryev V, Takada S, van Zonneveld AJ, Mano H, Plasterk R, Cuppen E (2006) Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis. Genome Res 16:1289–1298CrossRefPubMedGoogle Scholar
- Pfeffer S, Sewer A, Lagos-Quintana M, Sheridan R, Sander C, Grässer FA, van Dyk LF, Ho CK, Shuman S, Chien M, Russo JJ, Ju J, Randall G, Lindenbach BD, Rice CM, Simon V, Ho DD, Zavolan M, Tuschl T (2005) Identification of microRNAs of the herpesvirus family. Nat Methods 2:269–276CrossRefPubMedGoogle Scholar