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Molecular Genetics and Genomics

, Volume 284, Issue 2, pp 75–94 | Cite as

Centromere identity: a challenge to be faced

  • Gunjan D. Mehta
  • Meenakshi P. Agarwal
  • Santanu Kumar Ghosh
Review

Abstract

The centromere is a genetic locus, required for faithful chromosome segregation, where spindle fibers attach to the chromosome through kinetochore. Loss of centromere or formation of multiple centromeres on a single chromosome leads to chromosome missegregation or chromosome breakage, respectively, which are detrimental for fitness and survival of a cell. Therefore, understanding the mechanism of centromere locus determination on the chromosome and perpetuation of such a locus in subsequent generation (known as centromere identity) is very fundamental to combat conditions like aneuploidy, spontaneous abortion, developmental defects, cell lethality and cancer. Recent studies have come up with different models to explain centromere identity. However, the exact mechanism still remains elusive. It has been observed that most eukaryotic centromeres are determined epigenetically rather than by a DNA sequence. The epigenetic marks that are instrumental in determining centromere identity are the histone H3 variant, CENP-A and the specialized posttranslational modification of the core histones. Here we will review the recent studies on the factors responsible for generating unique centromeric chromatin and how it perpetuates during cell division giving the present-day models. We will further focus on the probable mechanism of de novo centromere formation with an example of neocentromere. As a matter of similitude, this review will include marking extrachromosomal chromatin to be served as a partitioning locus by deposition of CENP-A homolog in budding yeast.

Keywords

Centromere Epigenetics CENP-A Histone modification Chromosome 

Abbreviations

CENP-A

Centromeric protein A

CCAN

Constitutive centromere-associated network

CHD1

Chromodomain helicase DNA-binding protein 1

HJURP

Holliday junction recognition protein

KNL2

Kinetochore null phenotype

NURF

Nucleosome remodeling factor

NASP

Nuclear autoantigenic sperm protein

Notes

Acknowledgments

We acknowledge Sujata Hajra for a critical reading of the manuscript. We regret not being able to refer to the work of everyone in the field. We are grateful to the reviewers for their insightful critique that helped improve the article’s style and content. G.D.M. and M.P.A. are supported by CSIR fellowships (20-6/2009(i)EU-IV/329667, EU-IV/2008/JUNE/327214, respectively). SKG laboratory is supported by start-up grant from the Indian Institute of Technology, Bombay, India.

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Gunjan D. Mehta
    • 1
  • Meenakshi P. Agarwal
    • 1
  • Santanu Kumar Ghosh
    • 1
  1. 1.Department of Biosciences and BioengineeringIndian Institute of Technology BombayMumbaiIndia

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