Molecular Genetics and Genomics

, Volume 282, Issue 4, pp 437–451 | Cite as

Cyk3 acts in actomyosin ring independent cytokinesis by recruiting Inn1 to the yeast bud neck

  • Arne Jendretzki
  • Ivan Ciklic
  • Rosaura Rodicio
  • Hans-Peter Schmitz
  • Jürgen J. Heinisch
Original Paper

Abstract

Cytokinesis in yeast can be achieved by plasma membrane ingression, which is dependent on actomyosin ring constriction. Inn1 presumably couples these processes by interaction with both the plasma membrane and the temporary actomyosin ring component Hof1. In addition, an actomyosin ring independent cytokinesis pathway exists in yeast. We here identified Cyk3, a key component of the alternative pathway, as a novel interaction partner of Inn1. The carboxy-terminal proline rich part of Inn1 binds the SH3 domains of either Cyk3 or Hof1. Strains with truncated proteins lacking either of these SH3 domains do not display any severe phenotypes, but are synthetically lethal, demonstrating their crucial role in cytokinesis. Overexpression of CYK3 leads to an actomyosin ring independent recruitment of Inn1 to the bud neck, further supporting the significance of this interaction in vivo. Moreover, overexpression of CYK3 in a myo1 or an iqg1 deletion not only restores viability, but also the recruitment of Inn1 to the bud neck. We propose that Cyk3 is part of an actomyosin ring independent cytokinesis pathway, which acts as a rescue mechanism to recruit Inn1 to the bud neck.

Keywords

Hof1 Iqg1 Myo1 Inn1 Actomyosin ring Cytokinesis 

Notes

Acknowledgments

We thank Cornelia Broecker, Janina Wittland and Franziska Ahnert for experimental support and critical reading of the manuscript. We also thank Anja Lorberg and Christian Ungermann for providing specific antisera and for critical discussions. This work was supported by a grant from the Deutsche Forschungsgemeinschaft within the framework of the Graduiertenkolleg 612.

Supplementary material

438_2009_476_MOESM1_ESM.doc (41 kb)
Supplementary material 1 (DOC 41 kb)

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Arne Jendretzki
    • 1
  • Ivan Ciklic
    • 1
  • Rosaura Rodicio
    • 1
    • 2
  • Hans-Peter Schmitz
    • 1
  • Jürgen J. Heinisch
    • 1
  1. 1.Universität Osnabrück, Fachbereich Biologie/ChemieOsnabrückGermany
  2. 2.Departamento de Bioquímica y Biología Molecular and Instituto Universitario de Biotecnología de AsturiasUniversidad de OviedoOviedoSpain

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