Molecular Genetics and Genomics

, Volume 281, Issue 5, pp 511–523 | Cite as

Plc1p is required for proper chromatin structure and activity of the kinetochore in Saccharomyces cerevisiae by facilitating recruitment of the RSC complex

  • Parima Desai
  • Nilanjan Guha
  • Luciano Galdieri
  • Sara Hadi
  • Ales Vancura
Original Paper

Abstract

High-fidelity chromosome segregation during mitosis requires kinetochores, protein complexes that assemble on centromeric DNA and mediate chromosome attachment to spindle microtubules. In budding yeast, phosphoinositide-specific phospholipase C (Plc1p encoded by PLC1 gene) is important for function of kinetochores. Deletion of PLC1 results in alterations in chromatin structure of centromeres, reduced binding of microtubules to minichromosomes, and a higher frequency of chromosome loss. The mechanism of Plc1p’s involvement in kinetochore activity was not initially obvious; however, a testable hypothesis emerged with the discovery of the role of inositol polyphosphates (InsPs), produced by a Plc1p-dependent pathway, in the regulation of chromatin-remodeling complexes. In addition, the remodels structure of chromatin (RSC) chromatin-remodeling complex was found to associate with kinetochores and to affect centromeric chromatin structure. We report here that Plc1p and InsPs are required for recruitment of the RSC complex to kinetochores, which is important for establishing proper chromatin structure of centromeres and centromere proximal regions. Mutations in PLC1 and components of the RSC complex exhibit strong genetic interactions and display synthetic growth defect, altered nuclear morphology, and higher frequency of minichromosome loss. The results thus provide a mechanistic explanation for the previously elusive role of Plc1p and InsPs in kinetochore function.

Keywords

Phospholipase C RSC complex Chromatin structure Kinetochore function 

Notes

Acknowledgments

We thank Drs Baetz, Hieter, Laurent, Kaufman, Measday, Rine, Stillman, Struhl, Wente, and York for strains and plasmids and members of Vancura laboratory and Dr Vancurova for helpful comments. This work was supported by grants from the National Institutes of Health (GM076075) to A. Vancura.

Supplementary material

438_2009_427_MOESM1_ESM.pdf (22 kb)
Supplementary material 1 (PDF 21 kb)

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Parima Desai
    • 1
  • Nilanjan Guha
    • 1
  • Luciano Galdieri
    • 1
  • Sara Hadi
    • 1
  • Ales Vancura
    • 1
  1. 1.Department of Biological SciencesSt John’s UniversityQueensUSA

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