Molecular Genetics and Genomics

, Volume 281, Issue 1, pp 67–75 | Cite as

Cloning and characterization of the gene encoding the bovine BOULE protein

  • Qingbo Zhang
  • Jiahuang Li
  • Qifa Li
  • Xinfu Li
  • Zhenshan Liu
  • Dawei Song
  • Zhuang Xie
Original Paper

Abstract

The Deleted in Azoospermia (DAZ) genes encode potential RNA-binding proteins that are expressed exclusively in the germ-line. The bovine Deleted in Azoospermia-like gene is a strong candidate for male cattle-yak infertility. In this work, with the goe goal to further reveal the genetic cause of male cattle-yak sterility, another bovine DAZ family gene, b-boule, was isolated and characterized. The b-boule gene is predicted to encode a polypeptide of 295 amino acids with an RNP-type RNA recognition domain. Tertiary structure analysis shows that b-boule binds specifically to polypyrimidine RNAs and might act as a nuclear ribonucleoprotein particle auxiliary factor during germ cell formation and morphological changes of germ cells. RT-PCR assays revealed that b-boule was expressed specifically in the adult testis. However, an extremely low level of expression was detected in the testis of sterile male cattle-yaks. Microstructure of the testes from sterile males showed that type A spermatogonia were the only germ cells present and that few germ cells developed further than the stage of pachytene spermatocytes. These results suggest that b-boule may function in bovine spermatogenesis, and that low levels of b-boule expression might lead to male sterility in cattle-yaks.

Keywords

Cattle-yak Male sterility b-boule Spermatogenesis Protein structure 

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Qingbo Zhang
    • 1
  • Jiahuang Li
    • 2
  • Qifa Li
    • 1
  • Xinfu Li
    • 1
  • Zhenshan Liu
    • 1
  • Dawei Song
    • 1
  • Zhuang Xie
    • 1
  1. 1.Laboratory of Animal Reproductive Genetics and Molecular Evolution, College of Animal Science and TechnologyNanjing Agriculture UniversityNanjingPeople’s Republic of China
  2. 2.The State Key Laboratory of Pharmaceutical Biotechnology, College of Life ScienceNanjing UniversityNanjingPeople’s Republic of China

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