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Molecular Genetics and Genomics

, Volume 281, Issue 1, pp 43–54 | Cite as

An insertion of oleate desaturase homologous sequence silences via siRNA the functional gene leading to high oleic acid content in sunflower seed oil

  • Séverine Lacombe
  • Irénée Souyris
  • André J. Bervillé
Original Paper

Abstract

Classical sunflower varieties display a high linoleic acid content in their seeds [low oleic (LO) varieties] whereas genotypes carrying the Pervenets mutation display an increased oleic acid content of above 83% [high oleic (HO) varieties]. Despite the advantage in health terms of oleic acid, the nature of the mutation was still unknown. Previous work reported that HO genotypes carried a specific oleate desaturase (OD) allele. This enzyme catalyses the desaturation of oleic acid into linoleic acid. The present work demonstrates that this allele is organised in two parts: the first section present in both HO and LO genotypes carries a normal OD gene, the second section is specific to HO genotypes and carries OD duplications. The study of mRNA accumulation in LO and HO seeds revealed that the mutation is dominant and induces an OD mRNA down-regulation. Furthermore, OD small interfering RNA, characteristic of gene silencing, accumulated specifically in HO seeds. Considered together, these observations show that the mutation is associated with OD duplications leading to gene silencing of the OD gene and consequently, to oleic acid accumulation. This finding allowed the development of molecular markers characterising the mutation that can be used in breeding programmes to facilitate the selection of HO genotypes.

Keywords

High oleic acid oil Pervenets mutation Sunflower PTGS 

Notes

Acknowledgements

The authors wish to thank Lia Arraiano for the English editing of the manuscript. Séverine Lacombe’s fellowship was supported by MONSANTO within the frame of the contract B03984.

Supplementary material

438_2008_391_MOESM1_ESM.doc (38 kb)
MOESM1 (DOC 38 kb)
438_2008_391_MOESM2_ESM.doc (29 kb)
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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Séverine Lacombe
    • 1
    • 3
  • Irénée Souyris
    • 2
  • André J. Bervillé
    • 2
  1. 1.Monsanto SASPeyrehorade CedexFrance
  2. 2.Ressources Génétiques du tournesol, UMR-DIAPCINRAMontpellier Cedex 1France
  3. 3.UMR UP-IRD-CNRS 5096Centre IRDMontpellier Cedex 5France

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