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Molecular Genetics and Genomics

, Volume 279, Issue 5, pp 499–507 | Cite as

NARROW LEAF 7 controls leaf shape mediated by auxin in rice

  • Kenji Fujino
  • Yasuyuki Matsuda
  • Kenjirou Ozawa
  • Takeshi Nishimura
  • Tomokazu Koshiba
  • Marco W. Fraaije
  • Hiroshi Sekiguchi
Original Paper

Abstract

Elucidation of the genetic basis of the control of leaf shape could be of use in the manipulation of crop traits, leading to more stable and increased crop production. To improve our understanding of the process controlling leaf shape, we identified a mutant gene in rice that causes a significant decrease in the width of the leaf blade, termed narrow leaf 7 (nal7). This spontaneous mutation of nal7 occurred during the process of developing advanced backcrossed progeny derived from crosses of rice varieties with wild type leaf phenotype. While the mutation resulted in reduced leaf width, no significant morphological changes at the cellular level in leaves were observed, except in bulliform cells. The NAL7 locus encodes a flavin-containing monooxygenase, which displays sequence homology with YUCCA. Inspection of a structural model of NAL7 suggests that the mutation results in an inactive enzyme. The IAA content in the nal7 mutant was altered compared with that of wild type. The nal7 mutant overexpressing NAL7 cDNA exhibited overgrowth and abnormal morphology of the root, which was likely to be due to auxin overproduction. These results indicate that NAL7 is involved in auxin biosynthesis.

Keywords

Rice Narrow leaf FMO Auxin YUCCA 

Supplementary material

438_2008_328_MOESM1_ESM.tif (45 kb)
Frequency distribution of the width of the leaf blade in the flag leaf in the advanced backcrossed progeny. Arrowhead indicates the mean of Hayamasari (HY). Three classified genotypes, homozygous for the Italica Livorno allele (black), heterozygous (hatched), and homozygous for the Hayamasari allele (white), assessed using the marker GBR3003, are indicated (TIF 46 kb)
438_2008_328_MOESM2_ESM.tif (71 kb)
Sequence alignment of NAL7. The mutation identified in the nal7 mutant allele is indicated by bold type and by an arrowhead above the aligned sequences. The two conserved motifs are boxed (TIF 72 kb)
438_2008_328_MOESM3_ESM.tif (62 kb)
Phylogenetic tree of the YUCCA gene family. The phylogenetic tree of 14 rice and 11 Arabidopsis YUCCA genes was constructed using CLUSTAL W. Bootstrap analysis values are shown at the nodal branches. The indicated scale represents 0.1 amino acid substitution per site. The accession numbers and plant species are shown in green for Arabidopsis, blue for rice from Yamamoto et al. (2007), and red for rice from this study. Gene ID is according to RAP-DB (http://rapdb.lab.nig.ac.jp/index.html (TIF 62 kb)
438_2008_328_MOESM4_ESM.tif (158 kb)
Expression of nal7 in transgenic plants measured by RT-PCR analysis. Ten independent lines (T1), and Hayamasari and the nal7 mutant as non transgenic plants were used. Total RNA was extracted from the whole shoot of the 6-day-old seedling. The numbers on the right indicate the number of PCR cycles. Ubi2 was used as a loading control (TIF 158 kb)
438_2008_328_MOESM5_ESM.tif (39 kb)
Supplementary material Table S1 (TIF 69 kb)
438_2008_328_MOESM6_ESM.tif (39 kb)
Supplementary material Table S2 (TIF 70 kb)
438_2008_328_MOESM7_ESM.TIF.tif
Supplementary material Table S3 (TIF 59 kb)

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Kenji Fujino
    • 1
  • Yasuyuki Matsuda
    • 1
  • Kenjirou Ozawa
    • 2
  • Takeshi Nishimura
    • 3
  • Tomokazu Koshiba
    • 3
  • Marco W. Fraaije
    • 4
  • Hiroshi Sekiguchi
    • 1
  1. 1.Agricultural Research InstituteHOKUREN Federation of Agricultural CooperativesHokkaidoJapan
  2. 2.Department of Low-Temperature SciencesNational Agricultural Research Center for Hokkaido RegionSapporo, HokkaidoJapan
  3. 3.Department of Biological SciencesTokyo Metropolitan UniversityHachioji, TokyoJapan
  4. 4.Biochemical Laboratory, Groningen Biomolecular Sciences and Biotechnology InstituteUniversity of GroningenGroningenThe Netherlands

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