Molecular Genetics and Genomics

, Volume 279, Issue 3, pp 279–289 | Cite as

Identification, characterization and interaction of HAP family genes in rice

  • Thiruvengadam Thirumurugan
  • Yukihiro Ito
  • Takahiko Kubo
  • Akiko Serizawa
  • Nori Kurata
Original Paper

Abstract

A HAP complex, which consists of three subunits, namely HAP2 (also called NF-YA or CBF-B), HAP3 (NF-YB/CBF-A) and HAP5 (NF-YC/CBF-C), binds to CCAAT sequences in a promoter to control the expression of target genes. We identified 10 HAP2 genes, 11 HAP3 genes and 7 HAP5 genes in the rice genome. All the three HAP family genes encode a protein with a conserved domain in each family and various non-conserved regions in both length and amino acid sequence. These genes showed various expression patterns depending on genes, and various combinations of overlapped expression of the HAP2, HAP3 and HAP5 genes were observed. Furthermore, protein interaction analyses showed interaction of OsHAP3A, a ubiquitously expressed HAP3 subunit of rice, with specific members of HAP5. These results indicate that the formation of specific complex with various HAP subunits combinations can be achieved by both tissue specific expression of three subunit genes and specific interaction of three subunit proteins. This may suggest that the HAP complexes may control various aspects of rice growth and development through tissue specific expression and complex formation of three subunit members.

Keywords

CCAAT-box HAP2/NF-YA/CBF-B HAP3/NF-YB/CBF-A HAP5/NF-YC/CBF-C Protein–protein interaction Rice 

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Thiruvengadam Thirumurugan
    • 1
    • 2
  • Yukihiro Ito
    • 1
    • 3
  • Takahiko Kubo
    • 1
  • Akiko Serizawa
    • 1
  • Nori Kurata
    • 1
    • 2
  1. 1.Genetic Strains Research CenterNational Institute of GeneticsShizuoka-kenJapan
  2. 2.School of Life ScienceGraduate University for Advanced StudiesShizuoka-kenJapan
  3. 3.Graduate School of Agricultural ScienceTohoku UniversitySendaiJapan

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