Ascorbate peroxidase gene family in tomato: its identification and characterization
- 906 Downloads
The antioxidative response, where ascorbate peroxidase (APX) is a key enzyme, is an integral part of the plant tolerance response to environmental stresses. As a first step towards the study of the physiological role and the regulation of the members of the Apx gene family, the orthologs of the stress-sensitive cultivated tomato Solanum lycopersicum cv. M82 (Slm) and of the wild salt-tolerant species S. pennellii acc. Atico (Spa) were identified by utilizing the tomato EST database, and characterized. A redundant list of 16 virtual Apx transcripts and four singleton ESTs was shown to correspond to seven genuine Apx genes. The complete tomato Apx gene family is comprised of genes encoding three cytosolic, two peroxisomal, and two chloroplastic APXs. These genes attained differential regulatory patterns in various Slm organs. More detailed study of Apx1 and Apx2 genes, that are the products of a recent gene duplication event, shows that they have already attained differential regulation within and between Slm and Spa under control and stress conditions. It is also suggested that due to lineage-specific gene duplication and lose events, intricate phylogenetic relationships exist among the members of the Apx gene families.
KeywordsAscorbate peroxidase Gene duplication Gene expression Gene family Tomato
We thank Prof. L. Reinhold for critical reading of the manuscript and many useful comments. The study was supported by Dr. Herman Kessel Research Fund, in the memory of Mr. C. J. J. Van Kensbury.
- Corpas FJ, Gomez M, Hernandez JA, Delrio LA (1993) Metabolism of activated oxygen in peroxisomes from 2 pisum-sativum l cultivars with different sensitivity to sodium-chloride. J Plant Physiol 141:160–165Google Scholar
- Hillier LD, Lennon G, Becker M, Bonaldo MF, Chiapelli B, Chissoe S, Dietrich N, DuBuque T, Favello A, Gish W, Hawkins M, Hultman M, Kucaba M, Lacy M, Le M, Le N, Mardis E, Moore B, Morris M, Parsons J, Prange C, Rifkin L, Rohlfing T, Schellenberg K, Marra M (1996) Generation and analysis of 280,000 human expressed sequence tags. Genome Res 6:807–828PubMedCrossRefGoogle Scholar
- Meneguzzo S, Navari-Izzo F, Izzo R (1999) Antioxidative responses of shoots and roots of wheat to increasing NaCl concentrations. J Plant Physiol 155:274–280Google Scholar
- Mittova V, Theodoulou FL, Kiddle G, Volokita M, Tal M, Foyer CH, Guy M (2004) Comparison of mitochondrial ascorbate peroxidase in the cultivated tomato, Lycopersicon esculentum, and its wild, salt-tolerant relative, L. pennellii––a role for matrix isoforms in protection against oxidative damage. Plant Cell Environ 27:237–250CrossRefGoogle Scholar
- Narendra S, Venkataramani S, Shen GX, Wang J, Pasapula V, Lin Y, Kornyeyev D, Holaday AS, Zhang H (2006) The Arabidopsis ascorbate peroxidase 3 is a peroxisomal membrane-bound antioxidant enzyme and is dispensable for Arabidopsis growth and development. J Exp Bot 57:3033–3042PubMedCrossRefGoogle Scholar
- Rick CM (1979) Biosystematic studies in Lycopersicon and closely related species of Solanum. In: Hawkes JC, Lester RN, Skelding AD (eds) The biology of the Solanaceae. Academic, New York, pp 677–687Google Scholar
- Taha R, Mills D, Heimer Y, Tal M (2000) The relation between low K+/Na+ ratio and salt-tolerance in the wild tomato species Lycopersicon pennellii. J Plant Physiol 157:59–64Google Scholar
- Walker MA, McKersie BD (1993) Role of the ascorbate–glutathione antioxidant system in chilling resistance of tomato. J Plant Physiol 141:234–239Google Scholar