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Molecular Genetics and Genomics

, Volume 279, Issue 1, pp 95–106 | Cite as

Trans-specific S-RNase and SFB alleles in Prunus self-incompatibility haplotypes

  • Bruce G. Sutherland
  • Kenneth R. Tobutt
  • Timothy P. Robbins
Original Paper

Abstract

Self-incompatibility in the genus Prunus is controlled by two genes at the S-locus, S-RNase and SFB. Both genes exhibit the high polymorphism and high sequence diversity characteristic of plant self-incompatibility systems. Deduced polypeptide sequences of three myrobalan and three domestic plum S-RNases showed over 97% identity with S-RNases from other Prunus species, including almond, sweet cherry, Japanese apricot and Japanese plum. The second intron, which is generally highly polymorphic between alleles was also remarkably well conserved within these S-allele pairs. Degenerate consensus primers were developed and used to amplify and sequence the co-adapted polymorphic SFB alleles. Sequence comparisons also indicated high degrees of polypeptide sequence identity between three myrobalan and the three domestic plum SFB alleles and the corresponding Prunus SFB alleles. We discuss these trans-specific allele identities in terms of S-allele function, evolution of new allele specificities and Prunus taxonomy and speciation.

Keywords

Prunus Self-incompatibility SFB S-RNase Trans-specific evolution 

Notes

Acknowledgments

We thank Dr Encarna Ortega at CEBAS-CSIC (Spain) and Dr Simon Vaughan (EMR) for providing SFB allele sequence data for almond and sweet cherry. Thanks also to Emma-Jane Lamont at the National Fruit Collections at Brogdale (UK), Dr Encarna Ortega (CEBAS-CSIC) and Dr Yoshihiko Sato at the National Institute of Fruit Tree Science (Japan) for supplying Prunus material, and to Javier-Maria de Vera y Asensio for help with preparing the graphics. Bruce Sutherland gratefully acknowledges a PhD studentship from the University of Nottingham and the East Malling Trust for Horticultural Research.

Supplementary material

438_2007_300_MOESM1_ESM.doc (1.6 mb)
Supplementary data (DOC 19 kb)

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Bruce G. Sutherland
    • 1
    • 2
  • Kenneth R. Tobutt
    • 1
  • Timothy P. Robbins
    • 2
  1. 1.East Malling ResearchKentUK
  2. 2.Plant Science Division, School of BiosciencesUniversity of NottinghamLoughboroughUK

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