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Molecular Genetics and Genomics

, Volume 277, Issue 2, pp 115–137 | Cite as

Genome-wide expression profiling of ARABIDOPSIS RESPONSE REGULATOR 7(ARR7) overexpression in cytokinin response

  • Dong Ju Lee
  • Jin-Young Park
  • Su-Jin Ku
  • Young-Min Ha
  • Sunmi Kim
  • Myung Duk Kim
  • Man-Ho Oh
  • Jungmook KimEmail author
Original Paper

Abstract

The type-A ARRs of cytokinin two-component signaling system act as negative regulators for cytokinin signaling except for ARR4, but the molecular mechanism by which the A-type ARRs regulate cytokinin signaling remain elusive. To get insights into the molecular function of A-type ARR in cytokinin response, we sought to find the components that function downstream of A-type ARR protein by investigating the effects of ARR7 overexpression on cytokinin-regulated gene expression with the Affymetrix full genome array. To examine early cytokinin response, plants were treated with cytokinin for 30 min or 2 h, followed by GeneChip analysis. The hierarchical clustering analysis of our GeneChip data showed that ARR7 overexpression had distinctively repressive impacts on various groups of the cytokinin-regulated genes. In particular, the induction of all A-type ARRs except for ARR22, and AHK(ARABIDOPSIS HISTIDINE KINASE)1 and AHK4 was suppressed by ARR7. Cytokinin-induced expression of most of 12 expansin genes were repressed by ARR7, indicating potential involvement of ARR7 in cell expansion and plant development. Up-regulation of five cytokinin oxidase genes by cytokinins was negatively affected by ARR7. Our GeneChip analysis suggest that ARR7 mainly acts as a transcriptional repressor for a variety of early cytokinin-regulated genes encoding transcription factors, signal transmitters, plant development, and cellular metabolism, which may be responsible for reduced sensitivity of Arabidopsis transgenic plants overexpressing ARR7 to exogenous cytokinins.

Keywords

Arabidopsis response regulator Cytokinin signal transduction Expansin GeneChip Plant hormone 

Notes

Acknowledgments

This work was supported by grants from the Plant Diversity Research Center of 21st Century Frontier Research Program (PF0330404-02), funded by the Ministry of Science and Technology of Korean government and from the Agricultural Plant Stress Research Center (R11-20010003101-0) funded by Korea Science and Engineering Foundation to J. Kim. We thank Seoulin Bioscience for technical assistance with GeneChip analysis.

Supplementary material

438_2006_177_MOESM1_ESM.ppt (686 kb)
Supplementary material
438_2006_177_MOESM2_ESM.xls (34 kb)
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438_2006_177_MOESM3_ESM.xls (38 kb)
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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Dong Ju Lee
    • 1
  • Jin-Young Park
    • 1
  • Su-Jin Ku
    • 1
  • Young-Min Ha
    • 1
  • Sunmi Kim
    • 1
  • Myung Duk Kim
    • 1
  • Man-Ho Oh
    • 1
  • Jungmook Kim
    • 1
    Email author
  1. 1.Department of Plant Biotechnology and Agricultural Plant Stress Research CenterChonnam National UniversityGwangjuSouth Korea

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