Molecular Genetics and Genomics

, Volume 273, Issue 5, pp 382–393

Transcriptional profiling of Saccharomyces cerevisiae cells under adhesion-inducing conditions

  • Malte Kleinschmidt
  • Olav Grundmann
  • Nils Blüthgen
  • Hans-Ulrich Mösch
  • Gerhard H. Braus
Original Paper


The ability to adhere to other cells is one of the most prominent determinants of fungal pathogenicity. Thus, adherence of fungi to human tissues or plastics triggers hospital-acquired fungal infections, which are an increasing clinical problem, especially in immunocompromised persons. In the model fungus Saccharomyces cerevisiae adhesion can be induced by starvation for amino acids, and depends on the transcriptional activator of the general amino acid control system, Gcn4p. However, not much is known about the transcriptional program that mediates adhesive growth under such conditions. In this study, we present a genome-wide transcriptional analysis of Σ1278b yeast cells that were subjected to adhesion-inducing conditions imposed by amino acid starvation. Twenty-two novel genes were identified as inducible by amino acid starvation; 72 genes belonging to different functional groups, which were not previously known to be regulated by Gcn4p, require Gcn4p for full transcriptional induction under adhesion-inducing conditions. In addition, several genes were identified in Σ1278b cells that were inducible by amino acid starvation in a Gcn4p-independent manner. Our data suggest that adhesion of yeast cells induced by amino acid starvation is regulated by a complex, Σ1278b-specific transcriptional response.


Saccharomyces cerevisiae Transcriptional profiling Adhesion Gcn4 Amino acid starvation 

Supplementary material

Supplementary Fig. 1 Expression profiles of yeast cells under non-starvation conditions

438_2005_1139_ESM_supp_f1.pdf (161 kb)
(PDF 161 KB)

Supplementary Table 1 Condition GCN4 / gcn4

438_2005_1139_ESM_supp_t1.xls (700 kb)
(Excel 701 KB)

Supplementary Table 2 Condition wt + / - 10 mM 3AT; 8h

438_2005_1139_ESM_supp_t2.xls (664 kb)
(Excel 665 KB)

Supplementary Table 3 Condition gcn4 + / - 10 mM 3AT; 8h

438_2005_1139_ESM_supp_t3.xls (736 kb)
(Excel 736 KB)

Supplementary Table 4 Condition GCN4 + 10 mM 3AT / gcn4 + 10 mM 3AT; 8h

438_2005_1139_ESM_supp_t4.xls (730 kb)
(Excel 731 KB)

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Malte Kleinschmidt
    • 1
  • Olav Grundmann
    • 1
    • 3
  • Nils Blüthgen
    • 2
  • Hans-Ulrich Mösch
    • 1
    • 4
  • Gerhard H. Braus
    • 1
  1. 1.Institute of Microbiology and GeneticsGeorg-August-UniversityGöttingenGermany
  2. 2.Institute for Theoretical BiologyHumboldt-UniversityBerlinGermany
  3. 3.Department of MicrobiologyMax Planck Institute BremenBremenGermany
  4. 4.Department of GeneticsPhilipps-University MarburgMarburgGermany

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