Genetic mapping of expressed sequences in onion and in silico comparisons with rice show scant colinearity
- 450 Downloads
The Poales (which include the grasses) and Asparagales [which include onion (Allium cepa L.) and other Allium species] are the two most economically important monocot orders. Enormous genomic resources have been developed for the grasses; however, their applicability to other major monocot groups, such as the Asparagales, is unclear. Expressed sequence tags (ESTs) from onion that showed significant similarities (80% similarity over at least 70% of the sequence) to single positions in the rice genome were selected. One hundred new genetic markers developed from these ESTs were added to the intraspecific map derived from the BYG15-23×AC43 segregating family, producing 14 linkage groups encompassing 1,907 cM at LOD 4. Onion linkage groups were assigned to chromosomes using alien addition lines of Allium fistulosum L. carrying single onion chromosomes. Visual comparisons of genetic linkage in onion with physical linkage in rice revealed scant colinearity; however, short regions of colinearity could be identified. Our results demonstrate that the grasses may not be appropriate genomic models for other major monocot groups such as the Asparagales; this will make it necessary to develop genomic resources for these important plants.
KeywordsExpressed sequence tags (ESTs) Genetic markers Restriction fragment length polymorphisms (RFLPs) Simple sequence repeats (SSRs) Single nucleotide polymorphisms (SNPs)
This work was completed in compliance with the current laws governing genetic experimentation in Japan, New Zealand, and USA and was supported by U.S. Department of Agriculture, Initiative for Future Agriculture and Food Systems Grant 2001-04434 and Grant-in-Aid for Young Scientists (No. 40314827) from the Ministry of Education, Culture, Sports, Science and Technology, Japan.
Disclaimer: Names are necessary to report factually on available data; however, the U.S. Department of Agriculture (USDA) neither guarantees nor warrants the standard of the product, and the use of the name by USDA implies no approval of the product to the exclusion of others that may also be suitable.
- Arumuganathan K, Earle ED (1991) Nuclear DNA content of some important plant species. Plant Mol Biol Rep 9:208–218Google Scholar
- Chase M, Duvall M, Hills H, Conran J, Cox A, Eguiarte L, Hatwell K, Fay M, Caddick R, Cameron K, Hoot S (1995) Molecular systematics of Lilianae. In: Rudall P, Crib P, Culter D, Humphries C (eds) Monocotyledons: systematics and evolution. Royal Botanic Gardens, Kew, pp 109–137Google Scholar
- Chase MW, Soltis DE, Soltis PS, Rudall PJ, Fay MF, Hahn WH, Sullivan S, Joseph J, Molvray M, Kores PJ, Givnish TJ, Sytsma KJ, Pires JC (2000) Higher-level systematics of the monocotyledons: an assessment of current knowledge and a new classification. In: Wilson K, Morrison S (eds) Monocots: systematics and evolution (vol 1). CSIRO, Melbourne, pp 3–16Google Scholar
- FAO (2002) World production and trade statistics. http://apps.fao.org/.
- Kuhl JC, Cheung F, Yuan Q, Martin W, Zewdie Y, McCallum J, Catanach A, Rutherford P, Sink KC, Jenderek M, Prince JP, Town CD, Havey MJ (2004) A unique set of 11,008 onion (Allium cepa) ESTs reveals expressed sequence and genomic differences between monocot orders Asparagales and Poales. Plant Cell 16:114–125CrossRefPubMedGoogle Scholar
- Labani R, Elkington T (1987) Nuclear DNA variation in the genus Allium L. (Liliaceae). Heredity 59:119–128Google Scholar
- Maughan P, Saghai-Maroof M, Buss G (1996) Molecular-marker analysis of seed weight: genomic location, gene action, and evidence for orthologous evolution among three legume species. Theor Appl Genet 93:574–579Google Scholar
- Rudall P, Furness C, Chase M, Fay M (1997) Microsporogeneisis and pollen sulcus type in Asparagales (Lilianae). Can J Bot 75:408–430Google Scholar
- USDA (2002) Onion statistics. http://usda.mannlib.cornell.edu/reports/nassr/price
- Weeden N, Muehlbauer F, Ladizinsky G (1992) Extensive conservation of linkage relationships between pea and lentil genetic maps. J Hered 83:123–129Google Scholar
- Xin Z, Chen J, Burke J (2004) A low cost high efficient method for genetic mapping using indel markers. Poster abstract 227. Plant and Animal Genomes 12 Conference, San Diego. http://www.intl-pag.org/12/abstracts/P3b_PAG12_227.html