Molecular Genetics and Genomics

, Volume 272, Issue 4, pp 480–487 | Cite as

Isolation and diversity analysis of resistance gene analogues (RGAs) from cultivated and wild strawberries

  • M. G. Martínez Zamora
  • A. P. Castagnaro
  • J. C. Díaz Ricci
Original Paper

Abstract

Degenerate oligonucleotide primers, designed based on conserved regions of Nucleotide Binding Site (NBS) domains from previously cloned plant resistance genes, were used to isolate Resistance Gene Analogues (RGAs) from wild and cultivated strawberries. Seven distinct families of RGAs of the NBS-LRR type were identified from two related wild species, Fragaria vesca and F. chiloensis, and six different Fragaria × ananassa cultivars. With one exception (GAV-3), the deduced amino acid sequences of strawberry RGAs showed strong similarity to TIR (Toll Interleukin I Receptor)-type R genes from Arabidopsis, tobacco and flax, suggesting the existence of common ancestors. GAV-3 seemed to be more closely related to the non-TIR type. Further studies showed that the recombination level and the ratio of non-synonymous to synonymous substitutions within families were low. These data suggest that NBS-encoding sequences of RGAs in strawberry are subject to a gradual accumulation of mutations leading to purifying selection, rather than to a diversifying process. The present paper is the first report on RGAs in strawberry.

Keywords

Disease resistance genes Diversity NBS-LRR  Fragaria spp. Resistance Gene Analogues (RGAs) 

Notes

Acknowledgements

This paper was partially supported by grants PICT-7227, PICT-7229 from FONCYT and 26/D209 from CIUNT. Degenerate PCR primers were kindly provided by Dr. Fernando García-Arenal from ETSIA-Universidad Politécnica de Madrid, Spain. This work was carried out in compliance with the current laws regulating genetic experimentation in Argentina. GMZ is a fellow from CONICET and APC and JCDR are members of CONICET

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • M. G. Martínez Zamora
    • 1
  • A. P. Castagnaro
    • 2
  • J. C. Díaz Ricci
    • 1
  1. 1.Dpto. de Bioquímica de la Nutrición, Instituto de Química Biológica “Dr. Bernabé Bloj” (UNT)INSIBIO (CONICET-UNT)TucumánArgentina
  2. 2.Sección Biotecnología-Unidad asociada al INSIBIOEstación Experimental Agroindustrial O. ColombresTucumánArgentina

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