Molecular Genetics and Genomics

, Volume 272, Issue 2, pp 138–148

A survey of SL1-spliced transcripts from the root-lesion nematode Pratylenchus penetrans

  • M. Mitreva
  • A. A. Elling
  • M. Dante
  • A. P. Kloek
  • A. Kalyanaraman
  • S. Aluru
  • S W. Clifton
  • D. McK. Bird
  • T. J. Baum
  • J. P. McCarter
Original Paper

Abstract

Plant-parasitic nematodes are important and cosmopolitan pathogens of crops. Here, we describe the generation and analysis of 1928 expressed sequence tags (ESTs) of a splice-leader 1 (SL1) library from mixed life stages of the root-lesion nematode Pratylenchus penetrans. The ESTs were grouped into 420 clusters and classified by function using the Gene Ontology (GO) hierarchy and the Kyoto KEGG database. Approximately 80% of all translated clusters show homology to Caenorhabditis elegans proteins, and 37% of the C. elegans gene homologs had confirmed phenotypes as assessed by RNA interference tests. Use of an SL1-PCR approach, while ensuring the cloning of the 5′ ends of mRNAs, has demonstrated bias toward short transcripts. Putative nematode-specific and Pratylenchus -specific genes were identified, and their implications for nematode control strategies are discussed.

Keywords

Pratylenchus Expressed sequence tags (ESTs) Comparative genomics Gene expression Parasite 

Supplementary material

supp_tbls.pdf (252 kb)
sTables (PDF 252 KB)
supp_figs.pdf (24 kb)
sFigures (PDF 25 KB)

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • M. Mitreva
    • 1
  • A. A. Elling
    • 2
    • 3
  • M. Dante
    • 1
  • A. P. Kloek
    • 4
  • A. Kalyanaraman
    • 5
  • S. Aluru
    • 5
  • S W. Clifton
    • 1
  • D. McK. Bird
    • 6
  • T. J. Baum
    • 2
    • 3
  • J. P. McCarter
    • 1
    • 4
  1. 1.Genome Sequencing Center, Department of GeneticsWashington University School of MedicineSt. LouisUSA
  2. 2.Interdepartmental Genetics ProgramIowa State UniversityAmesUSA
  3. 3.Department of Plant PathologyIowa State UniversityAmesUSA
  4. 4.Divergence Inc.St. LouisUSA
  5. 5.Department of Electrical and Computer EngineeringIowa State UniversityAmesUSA
  6. 6.The Center for the Biology of Nematode ParasitismNorth Carolina State UniversityRaleighUSA

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