Molecular Genetics and Genomics

, Volume 272, Issue 1, pp 18–27 | Cite as

Pink (P), a new locus responsible for a pink trait in onions (Allium cepa) resulting from natural mutations of anthocyanidin synthase

  • S. Kim
  • M. L. Binzel
  • K. S. Yoo
  • S. Park
  • L. M. Pike
Original Paper
First Online:
Received:
Accepted:

Abstract

A new locus conditioning a pink trait in onions was identified. Unusual pink onions were found in haploid populations induced from an F1 hybrid between yellow and dark red parents and in F3 populations originating from the same cross. Segregation ratios of red to pink in F2, backcross, and F3 populations indicated that this pink trait is determined by a single recessive locus. RT-PCR was carried out to look for any differential expression of anthocyanin synthesis genes between dark red and pink F3 lines. The transcript level of anthocyanidin synthase (ANS) was significantly reduced in the pink line. To determine whether this reduced transcription is caused by other regulatory factors or by mutations in the ANS gene itself, ANS gene sequences from both dark red and pink F3 lines were compared to detect any polymorphisms. Polymorphisms were identified, and subsequently utilized as molecular markers for the selection of ANS alleles. Absolute co-segregation of the pink allele and the ANS allele from the pink line was observed in parents, F1 and F3 populations. These results indicate that reduced transcription of the ANS gene caused by mutations in a cis -acting element is likely to result in the pink trait in onions.

Keywords

Onion Anthocyanidin synthase (ANS) Bulb color inheritance Molecular marker 
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Notes

Acknowledgements

The authors thank members of Vegetable & Fruit Improvement Center for their dedicated support of this research. This work was supported by member contributions to the Vegetable & Fruit Improvement Center and by a U.S. Department of Agriculture grant (CSREES 2001-34402-10543, “Designing Foods for Health”).

References

  1. Alfenito MR, Souer E, Goodman CD, Buell R, Mol J, Koes R, Walbot V (1998) Functional complementation of anthocyanin sequestration in the vacuole by widely divergent glutathione S-transferases. Plant Cell 10:1135–1149CrossRefPubMedGoogle Scholar
  2. Bastianetto S, Quirion R (2002) Natural extracts as possible protective agents of brain aging. Neurobiol Aging 5687:1–7Google Scholar
  3. Bharti A, Khurana J (2003) Molecular characterization of transparent testa (tt) mutants of Arabidopsis thaliana (ecotype Estland) impaired in flavonoid biosynthesis pathway. Plant Sci 165:1321–1332CrossRefGoogle Scholar
  4. Bohanec B, Jakse M, Ihan A, Javornik B (1995) Studies of gynogenesis in onion (Allium cepa L.): induction procedures and genetic analysis of regenerants. Plant Sci 104:215–224CrossRefGoogle Scholar
  5. Boss P, Davies C, Robinson S (1996) Expression of anthocyanin biosynthesis pathway genes in red and white grape. Plant Mol Biol 32:565–569PubMedGoogle Scholar
  6. Braca A, Sortino C, Politi M, Morelli I, Mendez J (2002) Antioxidant activity of flavonoids from Licania licaniaeflora. J Ethnopharmacol 79:379–381CrossRefPubMedGoogle Scholar
  7. Campion B, Alloni C (1990) Induction of haploid plants in onion (Allium cepa L.) by in vitro culture of unpollinated ovules. Plant Cell Tiss Org Cult 20:1–6Google Scholar
  8. Campion B, Azzimonti MT, Vicini E, Schiavi M, Falavigna A (1992) Advances in haploid plant induction in onion (Allium cepa L.) through in vitro gynogenesis. Plant Sci 86:97–104CrossRefGoogle Scholar
  9. Clarke AE, Jones HA, Little TM (1944) Inheritance of bulb color in the onion. Genetics 29:569–575Google Scholar
  10. Cook NC, Samman S (1996) Flavonoids—chemistry, metabolism, cardioprotective effects, and dietary sources. Nutr Biochem 7:66–76Google Scholar
  11. Davis GN, El-Shafie MW (1967) Inheritance of bulb color in the onion (Allium cepa L.). Hilgardia 38:607–622Google Scholar
  12. Dooner HK, Robbins TP, Jorgensen RA (1991) Genetic and developmental control of anthocyanin biosynthesis. Annu Rev Genet 25:173–199CrossRefPubMedGoogle Scholar
  13. Falconer DS, Mackay TFC (1996) Introduction to quantitative genetics. Longman, London, pp 1–47Google Scholar
  14. Fossen T, Andersen OM, Ovstedal DO, Pedersen AT, Raknes A (1996) Characteristic anthocyanin pattern from onions and other Allium spp. J Food Sci 61:703–706Google Scholar
  15. Henikoff S, Henikoff G, Alford J, Pietrokovski S (1995) Automated construction and graphical presentation of protein blocks from unaligned sequences, Gene-COMBIS. Gene 163:GC17–26CrossRefPubMedGoogle Scholar
  16. Hollman P, Katan M (1997) Absorption, metabolism and health effects of dietary flavonoids in man. Biomed Pharmacother 51:305–310CrossRefPubMedGoogle Scholar
  17. Holton TA, Cornish EC (1995) Genetics and biochemistry of anthocyanin biosynthesis. Plant Cell 7:1070–1083CrossRefGoogle Scholar
  18. Keli S, Hertog M, Feskens E, Kromhout D (1996) Flavonoids, antioxidant vitamins and risk of stroke. The Zutphen study. Arch Int Med 156:637–642CrossRefGoogle Scholar
  19. Kim S, Binzel M, Yoo K, Park S, Pike L (2004) Inactivation of DFR (Dihydroflavonol 4-reductase) gene transcription results in blockage of anthocyanin production in yellow onions (Allium cepa). Mol Breeding, in pressGoogle Scholar
  20. Knekt P, Jarvinen R, Reunanen A, Maatela J (1996) Flavonoid intake and coronary mortality in Finland: a cohort study. Brit Med J 312:478–481PubMedGoogle Scholar
  21. Kobayashi T, Nakata T, Kuzumaki T (2002) Effect of flavonoids on cell cycle progression in prostate cancer cells. Cancer Lett 176:17–23CrossRefPubMedGoogle Scholar
  22. Larsen ES, Alfenito MR, Briggs WR, Walbot V (2003) A carnation anthocyanin mutant is complemented by the glutathione S-transferases encoded by maize Bz2 and petunia An9. Plant Cell Rep 21:900–904PubMedGoogle Scholar
  23. Li J, Ou-Lee T, Raba R, Amundson R, Last R (1993) Arabidopsis flavonoid mutants are hypersensitive to UV-B irradiation. Plant Cell 5:171–179CrossRefPubMedGoogle Scholar
  24. Marrs KA, Alfenito MR, Lloyd AM, Walbot V (1995) A glutathione S-transferase involved in vacuolar transfer encoded by the maize gene Bronze-2. Nature 375:397–400CrossRefPubMedGoogle Scholar
  25. Martin C, Prescott A, Mackay S, Bartlett J, Vrijlandt E (1991) Control of anthocyanin biosynthesis in flowers of Antirrhinum majus. Plant J 1:523–532CrossRefGoogle Scholar
  26. Menssen A, Höhmann S, Martin W, Schable PS, Peterson PA, Saedler H, Gierl A (1990) The En/Spm transposable element of Zea mays contains splice sites at the termini generating a novel intron from a dSpm element in the A2 gene. EMBO J 9:3051–3058PubMedGoogle Scholar
  27. Mol J, Jenkins G, Schafer E, Weiss D (1996) Signal perception, transduction, and gene expression involved in anthocyanin biosynthesis. Crit Rev Plant Sci 15:525–557Google Scholar
  28. Muren RC (1989) Haploid plant induction from unpollinated ovaries in onion. Hortscience 24:833–834Google Scholar
  29. Pike L (1986) Onion breeding. In: Basset MJ (ed) Breeding vegetable crops. AVI Publishing, Westport, Conn., pp 161–176Google Scholar
  30. Reiman GH (1931) Genetic factors for pigmentation in the onion and their relation to disease resistance. J Agr Res 42:251–278Google Scholar
  31. Rose M, Schultz R, Henikoff G, Pietrokovski S, McCallum M, Henikoff S (1998) Consensus-degenerate hybrid oligonucleotide primers for amplification of distantly-related sequences. Nucleic Acids Res 26:1628–1635CrossRefPubMedGoogle Scholar
  32. Shirley BW (1996) Flavonoid biosynthesis: ‘new’ functions for an ‘old’ pathway. Trends Plant Sci 1:377–382CrossRefGoogle Scholar
  33. Wilmouth R, Turnbull J, Welford R, Clifton I, Prescott A, Schofield C (2002) Structure and mechanism of anthocyanidin synthase from Arabidopsis thaliana. Structure 10:93–103CrossRefPubMedGoogle Scholar
  34. Yamazaki M, Makita Y, Springob K, Saito K (2003) Regulatory mechanisms for anthocyanin biosynthesis in chemotypes of Perilla frutescens var. crispa. Biochem Eng J 14:191-197CrossRefGoogle Scholar
  35. Zeback R, Dressler K, Hess D (1989) Flavonoid compounds from pollen and stigma of Petunia hybrida: inducers of the vir region of the Agrobacterium tumefaciens Ti plasmid. Plant Sci 62:83–91CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • S. Kim
    • 1
  • M. L. Binzel
    • 1
  • K. S. Yoo
    • 1
  • S. Park
    • 1
  • L. M. Pike
    • 1
  1. 1.Vegetable & Fruit Improvement Center, Department of Horticultural SciencesTexas A&M UniversityCollege StationUSA

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