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Molecular Genetics and Genomics

, Volume 272, Issue 2, pp 204–215 | Cite as

Identification of a novel two-partner secretion system from Burkholderia pseudomallei

  • N. F. Brown
  • C.-A. Logue
  • J. A. Boddey
  • R. Scott
  • R. G. Hirst
  • I. R. BeachamEmail author
Original Paper

Abstract

Two adjacent genes, bpaA and bpaB, whose products display significant similarity to a number of two-partner secretion (TPS) systems have been identified in Burkholderia pseudomallei strain 08, but are absent from the closely related avirulent species B. thailandensis. They possess a number of sequence features characteristic of TPS systems, including the presence of an NPNGI motif in a region of BpaA which strongly resembles a TPS secretion domain. BpaA is a very large protein (~530 kDa) and contains three repeats, each 600–800-amino acids long. Putative membrane-spanning regions in BpaB were identified through alignment with TpsB family members, and this also revealed an N-terminal extension not found in other TpsB proteins. The bpaA gene was found to be absent from the majority of B. pseudomallei strains. It appears that bpaAB are located within a putative genomic island that is inserted in close proximity to a methionine tRNACAT-encoding gene. Expression of BpaA was undetectable in cells grown in laboratory media. However, owing to the similarity of BpaA to known adhesin molecules, a potential role of BpaA in virulence was investigated in cell culture and in an animal model, but no evidence for such a role was found in these test systems.

Keywords

Burkholderia pseudomallei Secretion Deletion mutagenesis DNA sequence 

Notes

Acknowledgements

We thank Dr. Petra Oyston for providing plasmids. NFB and JAB are recipients of an Australian Postgraduate Award and C-AL holds a Griffith University Postgraduate Research Award

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • N. F. Brown
    • 1
    • 3
  • C.-A. Logue
    • 1
  • J. A. Boddey
    • 1
  • R. Scott
    • 2
  • R. G. Hirst
    • 2
  • I. R. Beacham
    • 1
    Email author
  1. 1.School of Health ScienceGriffith University-Gold Coast CampusGold CoastAustralia
  2. 2.Department of Microbiology and ImmunologyJames Cook UniversityTownsvilleAustralia
  3. 3.Biotechnology LaboratoryUniversity of British ColumbiaVancouverCanada

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