Molecular Genetics and Genomics

, Volume 270, Issue 2, pp 181–189 | Cite as

Serial Analysis of Gene Expression (SAGE) of Magnaporthe grisea: genes involved in appressorium formation

Original Paper

Abstract

Treatment with cyclic AMP (cAMP) induces appressorium formation in the phytopathogenic fungus Magnaporthe grisea, the causative agent of rice blast disease. In a search for the M. grisea genes responsible for appressorium formation and host invasion, SAGE (Serial Analysis of Gene Expression) was carried out using mRNA isolated from fungal conidia germinating in the presence and absence of cAMP. From cAMP-treated conidia 5087 tags including 2889 unique tags were isolated, whereas untreated conidia yielded 2342 unique tags out of total of 3938. cAMP treatment resulted in up- and down-regulation of genes corresponding to 57 and 53 unique tags, respectively. Upon consultation of EST/cDNA databases, 22 tags with higher representation in cAMP-treated conidia were annotated with putative gene names. Furthermore, 28 tags corresponding to cAMP-induced genes could be annotated with the help of the recently published genome sequence of M. grisea. cAMP-induced genes identified by SAGE included many genes that have not been described so far, as well as a number of genes known to be involved in pathogenicity, e.g. MPG1, MAS1 and MAC1. RT-PCR of 13 randomly selected genes confirmed the SAGE results, verifying the fidelity of the SAGE data.

Keywords

Magnaporthe grisea Rice blast SAGE (Serial Analysis of Gene Expression) Gene expression profile Appressorium 

Supplementary material

Table S1 SAGE results of cAMP- and non-treated M.grisea

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • T. Irie
    • 1
  • H. Matsumura
    • 2
  • R. Terauchi
    • 2
  • H. Saitoh
    • 2
    • 3
    • 4
  1. 1.Department of Environmental ScienceUniversity of Shiga PrefectureHikoneJapan
  2. 2.Iwate Biotechnology Research CenterKitakami, Iwate 024-0003Japan
  3. 3.Japan Society for the Promotion of ScienceChiyoda-ku, Tokyo 102-8471Japan
  4. 4.Max-Planck-Institute for Plant Breeding ResearchCologneGermany

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