Abstract
To gain an understanding of the molecular basis for resistance to rice blast ( Magnaporthe grisea), we have initiated a project to clone Pi5(t), a locus associated with broad-spectrum resistance to diverse blast isolates. AFLP-derived markers linked to Pi5(t)-mediated resistance were isolated using bulked segregant analysis of F2 populations generated by crossing three recombinant inbred lines (RILs), RIL125, RIL249, and RIL260 with the susceptible line CO39. The most tightly linked AFLP marker, S04G03, was positioned on chromosome 9 of the fingerprint-based physical map of Nipponbare, a well-characterized rice genotype. Flanking BAC-based Nipponbare markers were generated for saturation mapping using four populations, the three initial RILs and an additional one derived from a cross between M202 and RIL260. A BIBAC (binary BAC) library was constructed from RIL260. Using these resources Pi5(t) was mapped to a 170-kb interval, and a contiguous set of BIBAC clones spanning this region was constructed. It had previously been suggested that Pi3(t) and Pi5(t) might be allelic, due to their identical resistance spectrum and tight linkage. We therefore compared genomic regions for lines containing Pi3(t) using the Pi5(t)-linked markers. DNA gel-blot analyses indicated that the region around Pi3(t) is identical to that of Pi5(t), suggesting that Pi3(t) and Pi5(t) are the same resistance gene.
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Acknowledgements
We thank Deling Ruan, Patrik Canlas, Jin Fang and Erik Pogmore for technical assistance in the preparation of genomic DNAs. Masahiro Yano is specially acknowledged for mapping S04G03 on the Nipponbare/Kasalath genetic map. We are thankful to Matt Campbell, Kangle Zheng, Tsuyoshi Inukai, Kenong Xu, and Dave Mackill for helpful discussions. RFLP markers used in the study were kindly provided by Susan McCouch and Takuji Sasaki. We also thank Sally Leong and Gernot Presting for helpful comments on the use of the Nipponbare BAC clones, Rod Wing for providing Nipponabre BAC filters and clones, Hei Leung for providing RI lines, Shinji Kawasaki for providing the pBIGRZ vector, Dave Mackill for providing DNA filters of the Black Gora/Labell mapping population, and Harold Bockelman for providing C104PKT and Pia-Kan-Tao. We thank Todd Richter and Matt Campbell for critical reading of the manuscript. J.-S. Jeon was in part supported by the Korean Science and Engineering Foundation (KOSEF) and the Plant Metabolism Research Center (PMRC), Kyung Hee University, Korea. This project was supported by a United States of Department of Agriculture grant to P.C. Ronald
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Communicated by M.-A. Grandbastien
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Jeon, JS., Chen, D., Yi, GH. et al. Genetic and physical mapping of Pi5(t), a locus associated with broad-spectrum resistance to rice blast. Mol Gen Genomics 269, 280–289 (2003). https://doi.org/10.1007/s00438-003-0834-2
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DOI: https://doi.org/10.1007/s00438-003-0834-2