Molecular Genetics and Genomics

, Volume 267, Issue 4, pp 440–446 | Cite as

Direct interaction of the extracellular matrix protein DANCE with apolipoprotein(a) mediated by the kringle IV-type 2 domain

  •  A. Kapetanopoulos
  •  F. Fresser
  •  G. Millonig
  •  Y. Shaul
  •  G. Baier
  •  G. Utermann
Original Paper

Abstract.

Lipoprotein(a) [Lp(a)] consists of LDL and apolipoprotein(a), and has been shown to be a major, independent, risk factor for arteriosclerosis and thrombosis in humans. To further elucidate the (patho)physiological function(s) of Lp(a)/apo(a), we searched for new protein ligands, using the yeast two-hybrid system and employing the highly repetitive kringle IV type 2 (KIV-2) sequence from apo(a) as bait. The extracellular matrix protein DANCE [developmental arteries and neural crest epidermal growth factor (EGF)-like] recently implicated in atherogenesis was identified as an interactor. A direct physical interaction between DANCE and apo(a) was confirmed by co-purification of both recombinant proteins derived from culture supernatants of transiently transfected COS-1 cells. Furthermore, binding of human plasma-derived Lp(a) to recombinant DANCE was also observed. Finally, when monoclonal anti-apo(a) and polyclonal anti-DANCE antibodies were applied to tissue slices of atherosclerotic carotid artery, the two proteins were found to be co-localized in endothelial and smooth muscle cells, suggesting that they occur together in the arterial wall. However, as yet, the in vivo relevance and the possible functional role of this newly identified DANCE:Lp(a)/apo(a) interaction remains speculative.

Lipoprotein(a) Arteriosclerosis Yeast-two hybrid system Copurification Histochemistry. 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  •  A. Kapetanopoulos
    • 1
  •  F. Fresser
    • 1
  •  G. Millonig
    • 2
  •  Y. Shaul
    • 3
  •  G. Baier
    • 1
  •  G. Utermann
    • 1
  1. 1.Institute for Medical Biology and Human Genetics, University of Innsbruck, Schoepfstr. 41, A-6020 Innsbruck Austria
  2. 2.Institute of General and Experimental Pathology, University of Innsbruck, Fritz-Pregel-Str. 3, A-6020 Austria
  3. 3.Department of Molecular Genetics and Virology, Weizmann Institute of Science, Rehovot, Israel

Personalised recommendations