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Parasitology Research

, Volume 88, Issue 2, pp 144–149 | Cite as

Recombinant feline herpesvirus type 1 expressing Toxoplasma gondii ROP2 antigen inducible protective immunity in cats

  • Masayuki Mishima
  • Xuenan Xuan
  • Naoaki Yokoyama
  • Ikuo Igarashi
  • Kozo Fujisaki
  • Hideyuki Nagasawa
  • Takeshi Mikami
Original Paper

Abstract.

In order to investigate whether a recombinant viral vaccine composed of a feline herpesvirus type 1 (FHV1) vector and an immunogenic antigen of Toxoplasma gondii can induce a protective immunity in cats, a recombinant FHV1 expressing ROP2 antigen of T. gondii was prepared. We introduced a DNA fragment encoding 1–538 amino acid residues of ROP2 precursor into FHV1 genome under the control of a cytomegalovirus promoter. The recombinant FHV1 (FHV/ROP2) successfully expressed a 59 kDa antigen that was recognized by anti-ROP2 antibodies. Vaccination of cats with FHV/ROP2 induced serum IgG recognizing the native antigen. Moreover the antibodies inhibited the in vitro invasion of tachyzoites. The vaccination also accelerated the IgG response after T. gondii infection and reduced brain parasite load in cats.

Keywords

Toxoplasma Gondii Challenge Infection Recombinant Viral Vaccine Toxoplasma Infection Parasitophorous Vacuole Membrane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • Masayuki Mishima
    • 1
  • Xuenan Xuan
    • 1
  • Naoaki Yokoyama
    • 1
  • Ikuo Igarashi
    • 1
  • Kozo Fujisaki
    • 1
  • Hideyuki Nagasawa
    • 1
  • Takeshi Mikami
    • 1
  1. 1.National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, JapanJapan

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