Parasitology Research

, Volume 85, Issue 10, pp 800–808 | Cite as

Imaging Trypanosoma cruzi within tissues from chagasic patients using confocal microscopy with monoclonal antibodies

  • Renato Arruda Mortara
  • Solange da Silva
  • Francy R. Patrício
  • Maria de Lourdes Higuchi
  • Edison Reis Lopes
  • Alberto Alain Gabbai
  • Paola Carnevale
  • Ademir Rocha
  • Marcelo S. Ferreira
  • Márcia M. Souza
  • Marcelo F. de Franco
  • Gilberto Turcato Jr.
  • Ben-Hur Ferraz Neto
ORIGINAL PAPER

Abstract

Confocal fluorescence microscopy combined with differential interference contrast imaging of tissues from chagasic patients enabled the unequivocal identification of the parasite Trypanosoma cruzi. Using different monoclonal antibodies that indicate the parasite form and replication stage in conjunction with DNA labelling, specimens derived from distinct clinical forms of the disease were examined. Intracellular amastigote forms of the parasite were clearly detected in heart, brain, skin, lung, and kidney. Dividing amastigotes as well as trypomastigote forms were recognized in samples obtained from patients undergoing either acute-phase or some form of reactivation caused by immunosuppression.

Keywords

Monoclonal Antibody Fluorescence Microscopy Confocal Microscopy Contrast Imaging Trypanosoma 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • Renato Arruda Mortara
    • 1
  • Solange da Silva
    • 1
  • Francy R. Patrício
    • 2
  • Maria de Lourdes Higuchi
    • 3
  • Edison Reis Lopes
    • 4
  • Alberto Alain Gabbai
    • 5
  • Paola Carnevale
    • 6
  • Ademir Rocha
    • 7
  • Marcelo S. Ferreira
    • 7
  • Márcia M. Souza
    • 2
  • Marcelo F. de Franco
    • 2
  • Gilberto Turcato Jr.
    • 8
  • Ben-Hur Ferraz Neto
    • 9
  1. 1.Disciplina de Parasitologia, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, Escola Paulista de Medicina, R. Botucatu, 862, 6° andar, 04023-062, São Paulo, SP, Brazil e-mail: renato.dmip@epm.br, Tel.: +55-11-5708306, Fax: +55-11-5711095BR
  2. 2.Departamento de Anatomia Patológica, Universidade Federal de São Paulo, Escola Paulista de Medicina
  3. 3.Instituto do Coração, Faculdade de Medicina USP, São Paulo, SP, BrazilBR
  4. 4.Faculdade de Medicina do Triângulo Mineiro, Uberaba, MG, BrazilBR
  5. 5.Disciplina de Neurologia, Universidade Federal de São Paulo, Escola Paulista de Medicina
  6. 6.Disciplina de Cardiologia, Universidade Federal de São Paulo, Escola Paulista de Medicina
  7. 7.Centro de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, MG, BrazilBR
  8. 8.Departamento de Moléstias Infecciosas e Parasitárias, Universidade Federal de São Paulo, Escola Paulista de Medicina
  9. 9.Unifígado, Hospital Alemão Oswaldo Cruz, São Paulo, SP, BrazilBR

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