Parasitology Research

, Volume 85, Issue 8, pp 779–782

Chloramphenicol-sensitive mitochondrial translation in Trypanosoma brucei

  • Christoph E. Nabholz
  • Dave Speijer
  • André Schneider
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DOI: 10.1007/s004360050631

Cite this article as:
Nabholz, C., Speijer, D. & Schneider, A. Parasitol Res (1999) 85: 779. doi:10.1007/s004360050631

Abstract

We developed an in organello system to label newly synthesized mitochondrially encoded proteins of Trypanosoma brucei. Highly purified mitochondria, prepared under isotonic conditions, were incubated with radioactive methionine and cysteine in a suitable translation buffer. Analysis of mitochondrial extracts on TRIS-Tricine gels revealed a subset of labeled, NP-40-insoluble proteins. The labeling of these proteins was resistant to the cytosol-specific translation inhibitor cycloheximide. The proteins, however, were not labeled in the presence of chloramphenicol or erythromycin, inhibitors of prokaryotic type translation, or puromycin, a general translation inhibitor. These results indicate that isotonically isolated mitochondria of T. brucei are capable of protein synthesis.

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • Christoph E. Nabholz
    • 1
  • Dave Speijer
    • 2
  • André Schneider
    • 1
  1. 1.University of Fribourg, Institute of Zoology, Pérolles, CH-1700 Fribourg, Switzerland e-mail: andre.schneider@unifr.ch, Tel.: +41-26-300-8894, Fax: +41-26-300-9741CH
  2. 2.Protein Research Facility Amsterdam, E. C. Slater Instituut BioCentrum Amsterdam, The NetherlandsNL

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