Parasitology Research

, Volume 85, Issue 6, pp 452–456 | Cite as

Importance of the mutation of amino acid 200 of the isotype 1 β-tubulin gene in the benzimidazole resistance of the small-ruminant parasite Teladorsagia circumcincta

  • L. Elard
  • J. F. Humbert
ORIGINAL PAPER

Abstract

In this work we demonstrated that the acquisition of benzimidazole (BZ) resistance in the small- ruminant parasite Teladorsagia circumcincta is linked to the selection of individuals that are characterized by a tyrosine (Tyr) at amino acid 200 of their isotype 1 β-tubulin gene. This mutation appears to be recessive, since only homozygous mutant (Tyr/Tyr) individuals survived after BZ treatment of two resistant populations in which the three genotypes (rr, rs, ss) were initially present. In comparison with natural BZ-susceptible populations, a decrease in the restriction polymorphism (RFLP) of the isotype 1 β-tubulin gene was observed in natural resistant populations. It seems that this decrease in β-tubulin polymorphism results from the selection of homozygous mutant individuals.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • L. Elard
    • 1
  • J. F. Humbert
    • 2
  1. 1.INRA, Centre de Tours, Station de Pathologie Aviaire et de Parasitologie, F-37380 Nouzilly, FranceFR
  2. 2.INRA, Station d'Hydrobiologie Lacustre, 75 Av. de Corzent, BP 511, F-74203 Thonon, France E-mail: humbert@thonon.inra.fr Tel.: +33-4-50-26-78-09, Fax: +33-4-50-26-07-60FR

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